Structural Analysis of Human β-Defensin-1 and Its Significance in Urinary Tract Infection

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Abstract

Human β-defensin-1 (hBD-1) is a 36-amino-acid antimicrobial peptide that functions in the host innate defense. We developed a highly sensitive radioimmunoassay for hBD-1 and identified several hBD-1 peptides in human kidney, urine, and plasma by amino acid sequencing and mass spectrometry. Large quantities of hBD-1 peptides are produced in the kidney, are released into the tubular lumen as 47-amino-acid pro-hBD1, and then undergo proteolytic processing and generate multiple truncated forms. The respective urine and plasma concentrations of hBD-1 in patients with pyelonephritis are 48.1 ± (SEM) 15.7 pmol/mg creatinine and 2.66 ± 0.41 pmol/ml, 3.1-fold and 1.8-fold those of normal individuals. hBD-1 is thought to contribute to mucosal defense in the urinary tract. Our findings provide a better understanding of the biosynthesis of this peptide and its pathophysiological significance in infectious diseases.

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Most cited references 4

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A peptide antibiotic from human skin.

J. Harder, J. Bartels, E Christophers … (1997)

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hBD-1: a novel β-defensin from human plasma

Wolf-Georg Forssmann, Klaus W. Bensch, Manfred Raida … (1995)

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Localization of expression of human beta defensin-1 in the pancreas and kidney.

D Schnapp, C. Reid, A. Harris (1998)

Defensins are antimicrobial peptides which play a key role in innate immunity. High levels of human beta defensin-1 (hBD-1) have previously been detected in the kidney and pancreas, but the cell-specific location of hBD-1 mRNA has not been determined. The expression of hBD-1 mRNA has been examined in fetal and adult pancreas and kidney by mRNA in situ hybridization. In fetal pancreas, hBD-1 expression was detected in the developing acini and in adult pancreas in the acini, but not in the pancreatic ducts. In both fetal and adult kidney, hBD-1 expression was detected in the collecting ducts and in the loops of Henle in adult kidney. Expression of hBD-1 mRNA in the pancreas and kidney from early development and in the acini of the adult pancreas, rather than in the pancreatic ducts, may indicate that in these tissues, hBD-1 fulfils physiological functions in addition to host defence.

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Author and article information

Journal

Journal ID (publisher-id): NEF

Journal ID (nlm-ta): Nephron

Journal ID (doi): 10.1159/issn.1660-8151

Title: Nephron

Publisher: S. Karger AG

ISSN (Print): 1660-8151

ISSN (Electronic): 2235-3186

Publication date Subscription-year: 2000

Publication date (Print): May 2000

Publication date (Electronic): 21 April 2000

Volume: 85

Issue: 1

Pages: 34-40

Affiliations

^a3rd Department of Internal Medicine and ^bDepartment of Microbiology, Miyazaki Medical College, Miyazaki, ^cPeptide Institute, Inc., Osaka, ^dDepartment of Clinical Pathology, Osaka Medical College, Osaka, and ^eNational Cardiovascular Center Research Institute, Osaka, Japan

Article

Publisher ID: 45627 Other ID: Nephron 2000;85:34–40

DOI: 10.1159/000045627

PubMed ID: 10773753

SO-VID: 20bdd146-8f57-4b22-b916-9fdd61d9d021

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Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher. Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug. Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

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Structural Analysis of Human β-Defensin-1 and Its Significance in Urinary Tract Infection

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Abstract

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Karger: Nephrology

Most cited references 4

A peptide antibiotic from human skin.

hBD-1: a novel β-defensin from human plasma

Localization of expression of human beta defensin-1 in the pancreas and kidney.

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