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      Innervation of the Pars intermedia and Control of Alpha-Melanotropin Secretion in the Newt

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          Abstract

          In this study we have investigated the presence of nerve fibers containing dopamine, thyrotropin-releasing hormone (TRH) and neuropeptide Y (NPY) in the pars intermedia of the crested newt and we have examined the possible effect of these neurohormones on the release of alpha-melanotropin (alpha-MSH) by neurointermediate lobes in vitro. By means of immunohistochemistry, we observed the presence of tyrosine hydroxylase (TH)-immunoreactive fibers in the pars intermedia of the crested newt. Using a specific antiserum to dopamine, these fibers appeared to be mainly dopaminergic in nature. Unlike anurans, urodele amphibians do not exhibit TRH or NPY-like immunoreactivity in the pars intermedia. A perifusion system technique for newt pituitaries was developed to investigate the effect of dopamine, TRH and NPY on alpha-MSH secretion. Administration of increasing concentrations of dopamine (from 10<sup>–9</sup> to 10<sup>–5</sup> M) induced a dose-related inhibition of alpha-MSH release. This inhibitory effect was mimicked by the dopamine agonist apomorphine (10–<sup>6</sup>M). In contrast, the secretory activity of the newt pars intermedia was not affected by administration of synthetic TRH or NPY (up to 10<sup>–7</sup> and 10<sup>–6</sup> M, respectively). These results indicate that the neurotransmitter dopamine likely plays a pivotal role in the regulation of melanotropin secretion in urodele amphibians.

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          Author and article information

          Journal
          NEN
          Neuroendocrinology
          10.1159/issn.0028-3835
          Neuroendocrinology
          S. Karger AG
          0028-3835
          1423-0194
          1989
          1989
          02 April 2008
          : 50
          : 5
          : 543-549
          Affiliations
          aGroupe de Recherche en Endocrinologie Moléculaire, CNRS URA 650, Unité Alliée à l’INSERM, Université de Rouen, Mont-Saint-Aignan, France; bDipartimento di Biologia Animale, Torino; cDipartimento di Biologia Cellulare, Università della Calabria, Arcavacata di Rende, Cosenza, Italia; dINRS Santé, Université du Québec, Pointe-Claire, Canada
          Article
          125278 Neuroendocrinology 1989;50:543–549
          10.1159/000125278
          2514395
          © 1989 S. Karger AG, Basel

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          Page count
          Pages: 7
          Categories
          Original Paper

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