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      Identificación, biotipificación y caracterización de cepas de Pasteurella multocida aisladas en la Argentina Translated title: Identification, biotypification and characterization of Pasteurella multocida strains isolated in Argentina

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          Abstract

          Treinta cepas de Pasteurella multocida aisladas en la Argentina a partir de muestras de origen humano y animal fueron identificadas, biotipificadas y caracterizadas. Veintidós de ellas (73%) correspondieron a P. multocida subsp. multocida; cinco (17%) a P. multocida subsp. gallicida y tres (10%) a P. multocida subsp. septica. Todas las cepas fueron agrupadas en 8 biotipos; el 70% presentó el tipo capsular A. Los serotipos somáticos más frecuentes fueron el 1 (n:11) y el 3 (n:9). Las cepas de origen porcino fueron resistentes a tiamulina, estreptomicina y tetraciclina. La caracterización de las cepas de P. multocida aisladas en la Argentina es el primer paso para concretar futuros estudios destinados a la prevención y al tratamiento de la pasteurelosis en medicina humana y veterinaria.

          Translated abstract

          Thirty Pasteurella multocida strains isolated in Argentina from human and animal samples were identified, biotypified and characterized. Twenty-two (73%) strains were identified as P. multocida subsp. multocida, 5 (17%) as P. multocida subsp. gallicida, and 3 (10%) as P. multocida subsp. septica. All strains were grouped in 8 biotypes, and 70% of the strains presented capsular type A. The most frequent somatic serotypes were 1 (n:11) and 3 (n:9). P. multocida strains from swine source were resistant to tiamulin, streptomycin and tetracycline. Characterization of P. multocida strains isolated in Argentina is the first step to conduct future studies intended for the prevention and treatment of pasteurellosis in human and veterinary medicine.

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          Most cited references16

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          Genetic organization of Pasteurella multocida cap Loci and development of a multiplex capsular PCR typing system.

          Current serotyping methods classify Pasteurella multocida into five capsular serogroups (serogroups A, B, D, E, and F) and 16 somatic serotypes (serotypes 1 to 16). In the present study, we have developed a multiplex PCR assay as a rapid alternative to the conventional capsular serotyping system. The serogroup-specific primers used in this assay were designed following identification, sequence determination, and analysis of the capsular biosynthetic loci of each capsular serogroup. The entire capsular biosynthetic loci of P. multocida A:1 (X-73) and B:2 (M1404) have been cloned and sequenced previously (J. Y. Chung, Y. M. Zhang, and B. Adler, FEMS Microbiol. Lett. 166:289-296, 1998; J. D. Boyce, J. Y. Chung, and B. Adler, Vet. Microbiol. 72:121-134, 2000). Nucleotide sequence analysis of the biosynthetic region (region 2) from each of the remaining three serogroups, serogroups D, E, and F, identified serogroup-specific regions and gave an indication of the capsular polysaccharide composition. The multiplex capsular PCR assay was highly specific, and its results, with the exception of those for some serogroup F strains, correlated well with conventional serotyping results. Sequence analysis of the strains that gave conflicting results confirmed the validity of the multiplex PCR and indicated that these strains were in fact capsular serogroup A. The multiplex PCR will clarify the distinction between closely related serogroups A and F and constitutes a rapid assay for the definitive classification of P. multocida capsular types.
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            Fowl Cholera: Gel Diffusion Precipitin Test for Serotyping Pasteurella multocida from Avian Species

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              Antioxidants in Mediterranean diets.

              F Visioli (1999)
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                Author and article information

                Journal
                ram
                Revista argentina de microbiología
                Rev. argent. microbiol.
                Asociación Argentina de Microbiología (Ciudad Autónoma de Buenos Aires, , Argentina )
                0325-7541
                1851-7617
                September 2006
                : 38
                : 3
                : 125-129
                Affiliations
                [01] orgnameAdministración Nacional de Laboratorios e Institutos de Salud orgdiv1Instituto Nacional de Enfermedades Infecciosas orgdiv2Depatamento Bacteriología
                [02] Ciudad Autónoma de Buenos Aires orgnameAdministración Nacional de Laboratorios e Institutos de Salud orgdiv1Instituto Nacional de Enfermedades Infecciosas orgdiv2Departamento Bacteriología
                [03] La Plata Pcia. de Buenos Aires orgnameUniversidad Nacional de La Plata orgdiv1Facultad de Ciencias Veterinarias orgdiv2Laboratorio de Diagnóstico e Investigaciones Bacteriológicas Argentina
                Article
                S0325-75412006000300003 S0325-7541(06)03800300003
                214c43f6-c809-4f18-8ba9-083a92e4be4d

                This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

                History
                : 10 April 2006
                : 31 July 2006
                Page count
                Figures: 0, Tables: 0, Equations: 0, References: 14, Pages: 5
                Product

                SciELO Argentina

                Categories
                Microbiología Básica

                minimum inhibitory concentration,biochemical typing,Pasteurella multocida,PCR,pasteurellosis,concentración inhibitoria mínima,biotipificación,pasteurelosis

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