Basement Membrane Remodeling in Skeletal Muscles of Patients with Limb Ischemia Involves Regulation of Matrix Metalloproteinases and Tissue Inhibitor of Matrix Metalloproteinases

Abstract

Background/Aim: Because the pericapillary basement membrane in skeletal muscles of patients with chronic critical limb ischemia (CLI) is thickened, we determined the expression patterns of genes involved in collagen metabolism, using samples from 9 CLI patients, 4 patients with acute limb ischemia and 4 healthy controls. Methods: Gene array analysis, quantitative RT-PCR and semiquantitative grading of immunohistochemical reactivity were performed to determine mRNA/cDNA and protein concentrations. Results: In CLI patients compared to controls, cDNA levels of matrix metalloproteinase (MMP)-9 and MMP-19 were higher, collagen type IV chains A1 and A2, tissue inhibitor of matrix metalloproteinase (TIMP)-1 and TIMP-2 were similar and MMP-2 were lower. On the protein level, MMP-2, MMP-9, MMP-19 and TIMP-1 were more abundantly expressed. In skeletal muscles from patients with acute limb ischemia, cDNA and protein levels of MMP-9, MMP-19, collagen type IV chains, TIMP-1 and TIMP-2 were high. MMP-2 was elevated at the protein but decreased on the cDNA level. Conclusion: Expression of basement membrane components in skeletal muscles of CLI and acute limb ischemia patients is altered, possibly contributing to the pathogenesis of peripheral arterial disease.

Journal

Journal ID (publisher-id): JVR

Journal ID (nlm-ta): J Vasc Res

Journal ID (doi): 10.1159/issn.1018-1172

Title: Journal of Vascular Research

Publisher: S. Karger AG

ISSN (Print): 1018-1172

ISSN (Electronic): 1423-0135

Publication date Subscription-year: 2007

Publication date (Print): April 2007

Publication date (Electronic): 27 February 2007

Volume: 44

Issue: 3

Pages: 202-213

Affiliations

^aInstitute of Anatomy, University of Bern and ^bSwiss Cardiovascular Center, Division of Vascular Medicine, University Hospital of Bern, Inselspital, Bern, Switzerland; ^cCenter for Bioinformatics Tübingen, Wilhelm Schickard Institute, University of Tübingen, Tübingen, Germany

Article

Publisher ID: 100376 Other ID: J Vasc Res 2007;44:202–213

DOI: 10.1159/000100376

PubMed ID: 17337906

Copyright statement: © 2007 S. Karger AG, Basel

License:

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Page count

Figures: 5, Tables: 1, References: 35, Pages: 12

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Categories

Subject: Research Paper