Immunohistochemical Localization of Urotensin I/Corticotropin-Releasing Factor Immunoreactivity in Neurosecretory Neurons in the Caudal Spinal Cord of Fish

There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

Abstract

Urotensin I (UI), one of the biologically active peptides isolated from the caudal spinal cord and urophysis of fish, exhibits a strong sequence homology with mammalian corticotropin-releasing factor (CRF). We have applied an antiserum to ovine CRF that cross-reacts completely with UI to sections taken from the caudal spinal cord and urophysis of the channel catfish (Ictalurus punctatus). Using the indirect immunofluorescence technique, the presence of striking Ul-like immunoreactivity was observed within discrete neuronal structures. UI immunoreactivity was found within large-diameter neuronal perikarya in spinal cord segments immediately rostral to the urophysis, in fascicles of nerve fibers as the urophysis emerges, and in a dense plexus of nerve fibers and terminals which abut capillary loops within the urophysis. The localization of UI immunoreactivity within discrete neurosecretory neurons of this system will make it possible to more fully determine the nature of the regulatory mechanisms controlling its secretion.

Related collections

Author and article information

Journal

Journal ID (publisher-id): NEN

Journal ID (nlm-ta): Neuroendocrinology

Journal ID (doi): 10.1159/issn.0028-3835

Title: Neuroendocrinology

Publisher: S. Karger AG

ISSN (Print): 0028-3835

ISSN (Electronic): 1423-0194

Publication date Subscription-year: 1984

Publication date (Print): 1984

Publication date (Electronic): 28 March 2008

Volume: 39

Issue: 6

Pages: 503-509

Affiliations

Departments of ^aEcology and Behavioral Biology, and ^bAnatomy, University of Minnesota, Minneapolis, Minn., USA

Article

Publisher ID: 124030 Other ID: Neuroendocrinology 1984;39:503–509

DOI: 10.1159/000124030

PubMed ID: 6392919

SO-VID: 21dc19a8-f372-4ffc-be17-566d65d3be5a

License:

Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher. Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug. Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

History

Date received : 05 October 1983

Date accepted : 25 April 1984

Page count

Pages: 7

Comments

Comment on this article

scite_