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      Prolactin Receptor Immunoreactivity in Rat Anterior Pituitary

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          Abstract

          Prolactin (PRL) receptors have been identified in both classical and nonclassical target organs. Actions of PRL on the secretion of anterior pituitary hormones have been reported. In order to confirm the presence of PRL receptors on specific cell types of the anterior pituitary, immunocytology on ultrathin frozen sections was carried out using monoclonal antibodies specific to the rat PRL receptor. Anterior pituitary glands were removed and fixed in 4% paraformaldehyde or 2.5% glutaraldehyde and postfixed in 1% osmium tetroxide. Tissues were frozen and ultrathin sections were made by cryoultramicrotomy. In anterior pituitary cells, all endocrine cell populations showed PRL-R-like immunoreactivity. The relative labeling frequency based on immunolabeling studies was somatotrophs > lactotrophs > thyrotrophs > corticotrophs > gonadotrophs. Within all endocrine cell types, no apparent differences could be observed in the subcellular localization of PRL-R-like immunoreactivity. At the subcellular level, gold particles, either isolated or in groups of two, were seen at the level of the plasma membrane, in the cytoplasmic matrix, in the vicinity of the endoplasmic reticulum and in the nucleus.

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          Author and article information

          Journal
          NEN
          Neuroendocrinology
          10.1159/issn.0028-3835
          Neuroendocrinology
          S. Karger AG
          0028-3835
          1423-0194
          1994
          1994
          08 April 2008
          : 59
          : 1
          : 78-84
          Affiliations
          aCNRSURA 1454-Neuroendocrinologie, Faculté de Médécine Lyon-Sud, Oullins; bINSERM Unite 344, Endocrinologie Moléculaire, Faculté de Médécine Necker, Paris, France
          Article
          126641 Neuroendocrinology 1994;59:78–84
          10.1159/000126641
          8145897
          © 1994 S. Karger AG, Basel

          Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher. Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug. Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

          Page count
          Pages: 7
          Categories
          Growth Hormone and Prolactin Regulation

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