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      Identification of a novel Japanese flounder (Paralichthys olivaceus) CC chemokine gene and an analysis of its function.

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      Immunogenetics

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          Abstract

          A cDNA of Japanese flounder (Paralichthys olivaceus) CC chemokine designated as Paol-SCYA104 was cloned and sequenced. The cDNA contains an opening reading frame of 315 nucleotides encoding 104 amino acid residues. The full gene was cloned and sequenced from a BAC library. It has a length of approximately 750 bp from the start codon to the stop codon and is composed of four exons and three introns. Four cysteine residues are conserved in the same positions as those of mammalian and fish CC chemokines. Paol-SCYA104 gene was expressed in several organs, including peripheral blood leukocytes (PBLs), head kidney, trunk kidney, and spleen. The recombinant Paol-SCYA104 was expressed in Escherichia coli and the expressed protein was partially purified. The recombinant Paol-SCYA104 was able to attract Japanese flounder PBLs in a microchemotaxis chamber. On the other hand, a negative control, the fraction of the control cells carrying an expression vector lacking the Paol-SCYA104 cDNA, did not show chemotactic activity. These results indicate that Paol-SCYA104 probably acts as a CC chemokine.

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          Author and article information

          Journal
          Immunogenetics
          Immunogenetics
          0093-7711
          0093-7711
          Feb 2004
          : 55
          : 11
          Affiliations
          [1 ] Laboratory of Genome Science, Graduate School of Marine Science and Technology, Tokyo University of Marine Science and Technology, Konan 4-5-7, Minato, 108-8477 Tokyo, Japan.
          Article
          10.1007/s00251-003-0638-x
          14722688
          22ceae5e-b923-4b36-8d61-a0728ff30201
          History

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