Blog
About

27
views
0
recommends
+1 Recommend
0 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: found
      Is Open Access

      Chemical Analysis of the Chinese Liquor Luzhoulaojiao by Comprehensive Two-Dimensional Gas Chromatography/Time-of-Flight Mass Spectrometry

      Read this article at

      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          Luzhoulaojiao liquor is a type of Chinese liquor that dates back hundreds of years, but whose precise chemical composition remains unknown. This paper describes the screening of the liquor and the identification of its compounds using comprehensive two-dimensional gas chromatography/time-of-flight mass spectrometry (GC × GC/TOF-MS). Samples were prepared by both liquid-liquid extraction and solid-phase microextraction, which facilitated the detection of thousands of compounds in the liquor, thus demonstrating the superior performance of the proposed method over those reported in previous studies. A total of 320 compounds were common to all 18 types of Luzhoulaojiao liquor studied here, and 13 abundant and potentially bioactive compounds were further quantified. The results indicated that the high-performance method presented here is well suited for the detection and identification of compounds in liquors. This study also contributes to enriching our knowledge of the contents of Chinese liquors.

          Related collections

          Most cited references 19

          • Record: found
          • Abstract: found
          • Article: not found

          A synthetic mammalian gene circuit reveals antituberculosis compounds.

          Synthetic biology provides insight into natural gene-network dynamics and enables assembly of engineered transcription circuitries for production of difficult-to-access therapeutic molecules. In Mycobacterium tuberculosis EthR binds to a specific operator (O(ethR)) thereby repressing ethA and preventing EthA-catalyzed conversion of the prodrug ethionamide, which increases the resistance of the pathogen to this last-line-of-defense treatment. We have designed a synthetic mammalian gene circuit that senses the EthR-O(ethR) interaction in human cells and produces a quantitative reporter gene expression readout. Challenging of the synthetic network with compounds of a rationally designed chemical library revealed 2-phenylethyl-butyrate as a nontoxic substance that abolished EthR's repressor function inside human cells, in mice, and within M. tuberculosis where it triggered derepression of ethA and increased the sensitivity of this pathogen to ethionamide. The discovery of antituberculosis compounds by using synthetic mammalian gene circuits may establish a new line of defense against multidrug-resistant M. tuberculosis.
            Bookmark
            • Record: found
            • Abstract: found
            • Article: not found

            Recent developments in the application of comprehensive two-dimensional gas chromatography.

            The literature on comprehensive two-dimensional gas chromatography (GC x GC) is reviewed, with emphasis on application-oriented studies published in the period 2004-2006. The various strategies that can be used in such studies, the state-of-the-art analytical performance and the high potential of GC x GC combined with time-of-flight mass spectrometric detection are highlighted.
              Bookmark
              • Record: found
              • Abstract: found
              • Article: not found

              Characterization of flavor compounds in Chinese liquor Moutai by comprehensive two-dimensional gas chromatography/time-of-flight mass spectrometry.

              In this paper, a method to characterize the volatile compounds in Chinese liquors by comprehensive two-dimensional gas chromatography/time-of-flight mass spectrometry has been developed. The selection of column combination and the optimization of temperature program are discussed. With the optimized conditions, ordered chromatograms are achieved in the two-dimensional plane, which is useful for the identification of unknown compounds. According to the automated data processing by TOFMS software, combining with the ordered chromatogram and the retention index database developed by our group, a total of 528 components are identified in a Moutai liquor sample, including organic acids, alcohols, esters, ketones, aldehydes, acetals, lactones, nitrogen-containing and sulfur-containing compounds, etc. In addition, the contribution of some important aroma compounds to flavor of Moutai liquor has also been studied.
                Bookmark

                Author and article information

                Journal
                Sci Rep
                Sci Rep
                Scientific Reports
                Nature Publishing Group
                2045-2322
                10 April 2015
                2015
                : 5
                Affiliations
                [1 ]State Key Laboratory of Microbial Metabolism, and School of Life Sciences & Biotechnology, Shanghai Jiao Tong University , Shanghai 200240, People's Republic of China
                [2 ]National Engineering Research Center of Solid-State Brewing , Luzhou 646000, People's Republic of China
                [3 ]The Instrumental Analysis Center, Shanghai Jiao Tong University , Shanghai 200240, People's Republic of China
                Author notes
                Article
                srep09553
                10.1038/srep09553
                4392506
                25857434
                Copyright © 2015, Macmillan Publishers Limited. All rights reserved

                This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

                Categories
                Article

                Uncategorized

                Comments

                Comment on this article