4
views
0
recommends
+1 Recommend
0 collections
    0
    shares
      • Record: found
      • Abstract: not found
      • Article: not found

      Consensus Statement on Ehrlichial Disease of Small Animals from the Infectious Disease Study Group of the ACVIM

      , , ,
      Journal of Veterinary Internal Medicine
      Wiley

      Read this article at

      ScienceOpenPublisherPubMed
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Related collections

          Most cited references29

          • Record: found
          • Abstract: found
          • Article: not found

          PCR detection of acute Ehrlichia canis infection in dogs.

          A polymerase chain reaction (PCR)-based detection assay that specifically detected Ehrlichia canis in dogs with acute infections was developed. A region of the 16S ribosomal RNA gene of E. canis was targeted for PCR amplification and chemiluminescent hybridization (CH) with a complementary internal 287-base pair (bp) oligonucleotide probe. The CH improved the PCR assay sensitivity 1,000-fold as compared with visualization on ethidium bromide-stained agarose gels. The PCR assay with CH (PCR/CH) detected as little as 30 fg of E. canis genomic DNA, the equivalent of approximately 150 E. canis organisms. The 495-bp product defined by the specific primers was not detected when genomic DNA from E. platys, E. chaffeensis, E. risticii, and E. equi were used in the PCR/CH assay. The PCR/CH assay was tested with unfractionated blood samples collected from 9 dogs experimentally infected with E. canis. The PCR/CH assay had greater detection sensitivity than did cell culture isolation (CCI) from infected blood. PCR/CH detected E. canis 7 days prior to CCI in 4 of 6 experimentally infected dogs. The results obtained with the PCR/CH assay otherwise consistently matched the results obtained by CCI. This PCR/CH assay is a rapid, sensitive, and specific method for E. canis detection with sensitivity comparable to or exceeding that of CCI. A diagnosis of E. canis using this PCR/CH assay can be made in 2 days as compared with 1-4 weeks for CCI. The PCR/CH assay appears to be an acceptable alternative or complement to current diagnostic techniques.
            Bookmark
            • Record: found
            • Abstract: not found
            • Article: not found

            Exposure to deer blood may be a cause of human granulocytic ehrlichiosis.

              Bookmark
              • Record: found
              • Abstract: not found
              • Article: not found

              Ehrlichial Diseases of Dogs

                Bookmark

                Author and article information

                Journal
                JVIM
                Journal of Veterinary Internal Medicine
                Wiley
                08916640
                19391676
                May 2002
                May 2002
                : 16
                : 3
                : 309-315
                Article
                10.1111/j.1939-1676.2002.tb02374.x
                12041661
                23187360-92bf-4129-8bf0-2a7aa4db8c68
                © 2002

                http://doi.wiley.com/10.1002/tdm_license_1.1

                History

                Comments

                Comment on this article