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Ten blood samples randomly collected from cows on a farm nearby Antwerp, Belgium,
were inoculated into KIVI culture medium (Kit for In Vitro Isolation of trypanosomes)
and RPMI 10%+feeder medium. Within 3 weeks of incubation all KIVI cultures and four
RPMI 10%+feeder revealed presence of Trypanosoma theileri. Some practical implications
regarding the use of KIVI for isolation of pathogenic African trypanosomes from cattle
and other Bovidae are discussed.