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      Moutan Cortex and Paeoniae Radix Rubra reverse high-fat-diet-induced metabolic disorder and restore gut microbiota homeostasis

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          Abstract

          The present study was designed to investigate the therapeutic effcts of Moutan Cortex (CM, root bark of Paeonia suffruticosa Andr) and Paeoniae Radix Rubra (PR, root of Paeonia veitchii Lynch) on metabolic disorders, focusing on the infuence of CM and PR on the obesity-related gut microbiota homeostasis. The diet-induced obese (DIO) mouse model was used to test the therapeutic effects of CM and PR. The mice were orally administered with CM and PR for 6 weeks, and oral glucose tolerance test (OGTT) and insulin tolerance test (ITT) were performed to evaluate the insulin sensitivity of the mice. Sterol-regulatory element binding proteins (SREBPs) and their target genes were measured by quantitative RT-PCR. High-throughput 16S ribosomal RNA (16S rRNA) gene sequencing technology was used to determine the composition of gut microbiota, and the metabolites in serum were analyzed by GC-MS. Our results indicated that CM and PR combination alleviated obese and insulin resistance in the DIO mice, leading to increased glucose uptake and gene expression in muscle and liver, and down-regulated SREBPs and their target genes in liver. Interesting, neither the CM-PR extracts, nor the major components of CM and PR did not affect SREBPs activity in cultured cells. Meanwhile, CM and PR significantly modulated the gut microbiota of the high-fat diet (HFD) treated mice, similar to metformin, and CM-PR reversed the overall microbiota composition similar to the normal chow diet (NCD) treated mice. In conclusion, our results provide novel mechanisms of action for the effects of CM and PR in treating DIO-induced dysregulation of sugar and lipid metabolism.

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          Author and article information

          Journal
          CJNM
          Chinese Journal of Natural Medicines
          Elsevier
          1875-5364
          20 March 2017
          : 15
          : 3
          : 210-219
          Affiliations
          1State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, China
          2Jiangsu Key Laboratory of Drug Discovery for Metabolic Diseases, China Pharmaceutical University, Nanjing 210009, China
          Author notes
          *Corresponding authors: LI Ping, Tel/Fax: 86-25-83271379, E-mail: liping2004@ 123456126.com ; XU Xiao-Jun, Tel: 86-25-83271382, E-mail: xiaojunxu2000@ 123456163.com

          These authors have no conflict of interest to declare.

          Article
          S1875-5364(17)30037-7
          10.1016/S1875-5364(17)30037-7
          28411689
          Copyright © 2017 China Pharmaceutical University. Published by Elsevier B.V. All rights reserved.
          Funding
          Funded by: Fund for Creative Research Groups of China, National Natural Science Foundation of China
          Award ID: 81421005
          Funded by: Specialized Research Fund of the Doctoral Program of Higher Education (SRFDP) and Research Council Earmarked Research Grants (RCERG)
          Award ID: 201300 96140001
          Funded by: National Natural Science Foundation of China
          Award ID: 81274159
          Funded by: XU Xiao-Jun was supported by New Century Excellent Talents in University
          Award ID: NCET-12 0976
          This work was supported by the Fund for Creative Research Groups of China, National Natural Science Foundation of China (No. 81421005), the Specialized Research Fund of the Doctoral Program of Higher Education (SRFDP) and Research Council Earmarked Research Grants (RCERG) (No. 201300 96140001), and the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD). This work was also partially supported by the National Natural Science Foundation of China (No. 81274159). XU Xiao-Jun was supported by New Century Excellent Talents in University (NCET-12-0976).

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