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      Cinética de crecimiento y composición química del micelio de Lentinula edodes cultivado en medio líquido suplementado con extractos de madera de vid Translated title: Growth kinetics and chemical composition of Lentinula edodes mycelia produced in liquid medium supplemented with vineyard pruning extracts

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          Abstract

          En este estudio se evaluó la producción de biomasa, la cinética de crecimiento y la composición química del micelio de Lentinula edodes obtenido en cultivo sumergido con suplementación de extractos de madera de vid. Se evaluaron las cepas IE-245 e IE-105 de L. edodes y tres medios de cultivo base para la producción de biomasa. La combinación con mayor producción se eligió como control y base para formular dos tratamientos (T1: suplementado con los cuatro extractos y T2: suplementado con los extractos acuoso y etanólico). La producción de biomasa no varió significativamente entre los tratamientos (P>0.05), después de 21 días de cultivo. La máxima producción de biomasa predicha por el modelo logístico se observó en T2 -1-1(16.57 g L-1) y la mínima en el control (10.78 g L-1). Las µM para el control, T1 y T2 fueron 0.052, M-12 0.146 y 0.133 días-1, respectivamente. El control presentó la R² más baja (0.86), mientras que T2 la más alta (0.94). Se encontraron diferencias (P<0.05) entre el control y T1 en contenido de grasa cruda, así como entre el control y los tratamientos (T1 y T2) en los valores de proteínas y carbohidratos totales.

          Translated abstract

          The aim of this study was to evaluate the biomass production, the kinetic growth parameters and the chemical composition of Lentinula edodes mycelia produced in submerged cultures supplemented with vineyard prunning extracts (aqueous, ethanol, chloroform and toluene). Strains IE-105 and IE-245 of L. edodes and three basic culture media were evaluated for the biomass production. The combination with higher production was chosen as control and used for the design of two treatments (T1: supplemented with four extracts, and T2: supplemented with aqueous and ethanol extracts). Biomass production was not significantly different (P>0.05) among treatments after 21 days of fermentation process. The maximum biomass production predicted by the logistic model was observed in T2 (16.57 g L-1), and the -1lowest in the control (10.78 g L-1). The µM for the control, T1 and T2 were 0.052, 0.146, and 0.133 days-1, respectively. The lower R² was found in the control (0.86) and the higher in T2 (0.94). Differences were found (P<0.05) in the content of crude fat between control and T1, and in the amount of protein and total carbohydrates between treatments (T1 y T2) and control.

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          Most cited references38

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          Servicio de Información Agroalimentaria y Pesquera

          (2013)
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            Antioxidant and antimicrobial activities of shiitake (Lentinula edodes) extracts obtained by organic solvents and supercritical fluids

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              Antiinflammatory and Immunomodulating Properties of Fungal Metabolites

              We discuss current information on the ability of extracts and isolated metabolites from mushrooms to modulate immune responses. This can result in a more enhanced innate and acquired disease resistance. The major immunomodulating effects of these active substances derived from mushrooms include mitogenicity and activation of immune effector cells, such as lymphocytes, macrophages, and natural killer cells, resulting in the production of cytokines, including interleukins (ILs), tumor necrosis factor alpha (TNF)-α, and interferon gamma (INF)-γ. In particular, the ability of selective mushroom extracts to modulate the differentiation capacity of CD4+ T cells to mature into TH1 and/or TH2 subsets will be discussed. As a consequence these extracts will have profound effects in particular diseases, like chronic autoimmune TH1-mediated or allergic TH2-mediated diseases. Immunosuppressive effects by mushroom components have also been observed. The therapeutic effects of mushrooms, such as anticancer activity, suppression of autoimmune diseases, and allergy have been associated with their immunomodulating effects. However, further studies are needed to determine the molecular mechanisms of the immunomodulating effects of mushrooms metabolites both individually and in complex mixtures, for example, extracts.
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                Author and article information

                Journal
                rmm
                Revista mexicana de micología
                Rev. Mex. Mic
                Sociedad Mexicana de Micología (Xalapa, Veracruz, Mexico )
                0187-3180
                2013
                : 37
                : 51-59
                Affiliations
                [01] Hermosillo Sonora orgnameCentro de Investigación en Alimentación y Desarrollo, A.C. México esqueda@ 123456ciad.mx
                Article
                S0187-31802013000100007 S0187-3180(13)03700000007
                23a5647b-5305-4656-8b20-d029a8a35466

                This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

                History
                : 29 March 2013
                : 14 August 2012
                Page count
                Figures: 0, Tables: 0, Equations: 0, References: 28, Pages: 9
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                SciELO Mexico

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                Contribuciones

                shiitake,cultivo sumergido,producción de biomasa,composición proximal,submerged culture,biomass production,proximal composition

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