The immunodominant antigen A, IsaA, of Staphylococcus aureus is considered to be a sequence homologue of gram-negative soluble lytic transglycosylase. Recently, it was reported that IsaA production is probably regulated by the essential two-component regulatory system consisting of YycG and YycF. Therefore, we investigated the isaA expression profile by quantifying its gene products at different stages of bacterial growth. In the culture supernatant, IsaA was detectable in the early exponential growth phase and its concentration constantly increased until the early stationary phase. Cell surface IsaA was mainly found on growing bacteria, and disappeared proportionately with a decline in cell proliferation. On the other hand, the isaA transcript rapidly increased at the beginning of the culture, and then stayed at a constant level until the late exponential growth phase. These findings indicated that isaA gene expression was stimulated during the exponential growth phase and repressed in the stationary phase. Thus, IsaA production was associated with active bacterial growth. Our results support the previous report that isaA expression is regulated by YycF, and also suggest that IsaA is probably involved in bacterial proliferation by residing on the cell wall. We also discuss the possibility that isaA transcription may be controlled by SarA.