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      Delineation of BmSXP antibody V-gene usage from a lymphatic filariasis based immune scFv antibody library.

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          Abstract

          Phage display technology is an important tool for antibody generation or selection. This study describes the development of a scFv library and the subsequent analysis of identified monoclonal antibodies against BmSXP, a recombinant antigen for lymphatic filariasis. The immune library was generated from blood of lymphatic filariasis infected individuals. A TA based intermediary cloning approach was used to increase cloning efficiency for the library construction process. A diverse immune scFv library of 10(8) was generated. Six unique monoclonal antibodies were identified from the 50 isolated clones against BmSXP. Analysis of the clones showed a bias for the IgHV3 and Vκ1 (45.5%) and IgHV2 and Vκ3 (27.3%) gene family. The most favored J segment for light chain is IgKJ1 (45.5%). The most favored D and J segment for heavy chain are IgHD6-13 (75%) and IgHJ3 (47.7%). The information may suggest a predisposition of certain V genes in antibody responses against lymphatic filariasis.

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          Author and article information

          Journal
          Mol. Immunol.
          Molecular immunology
          Elsevier BV
          1872-9142
          0161-5890
          Oct 2015
          : 67
          : 2 Pt B
          Affiliations
          [1 ] Institute for Research in Molecular Medicine (INFORMM), Universiti Sains Malaysia, 11800 Minden, Penang, Malaysia.
          [2 ] Institute for Research in Molecular Medicine (INFORMM), Universiti Sains Malaysia, 11800 Minden, Penang, Malaysia. Electronic address: theamsoon@usm.my.
          Article
          S0161-5890(15)30048-1
          10.1016/j.molimm.2015.07.040
          26277276
          23fa255b-8782-4772-95df-50bd9127f534
          History

          Phage display,Lymphatic filariasis,Immune antibody library,V-gene usage

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