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      Perfiles proteicos de estados de desarrollo de Gonipterus scutellatus (Coleoptera: Curculionidae) Translated title: Protein profiles of Gonipterus scutellatus (Coleoptera: Curculionidae) development stages

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          Abstract

          En 1998 se detectó en Chile al gorgojo del eucalipto, Gonipterus scutellatus (Gyllenhal, 1833) (Coleoptera: Curculionidae), uno de los principales defoliadores del género Eucalyptus, que causa pérdidas de crecimiento y económicas. Esto ha generado preocupación pues hay más de 525.000 ha plantadas con especies de eucaliptos. Con el objetivo de conocer la respuesta de las proteínas de estados de desarrollo de G. scutellatus a las tres especies de eucaliptos más importantes en el país, se obtuvieron y caracterizaron perfiles electroforéticos en geles de poliacrilamida bajo condiciones desnaturalizantes, y se buscaron proteínas marcadoras por densitometría de geles. Las proteínas se hicieron más numerosas a medida que los insectos se desarrollan. Las bandas de proteínas se concentraron entre los 20 y 120 kDa, con excepción de los huevos, los que presentaron valores menores. La aparición de distintas bandas de proteínas en los extractos de larvas y adultos de G. scutellatus alimentados con distintas especies de eucaliptos reflejó también cambios en su metabolismo. Estos antecedentes son útiles para el manejo integrado de plagas debido a que la identificación de proteínas marcadoras posiblemente está asociada a patrones metabólicos relacionados con el consumo de las plantas hospederas. Los cambios en la dieta del insecto podrían incidir en las proteínas marcadoras, indicando una habilidad diferencial de G. scutellatus para usar las plantas hospederas.

          Translated abstract

          In 1998 the eucalyptus weevil, Gonipterus scutellatus (Coleoptera: Curculionidae), a major defoliator of the genus Eucalyptus, which causes growth and economic losses, was detected in Chile. This has caused concern because more than 525,000 ha are planted with species of eucalypt. To understand the protein responses of development stages of G. scutellatus fed on the three species of eucalypt trees most important in the country, electrophoretic profiles were obtained and characterized on polyacrylamide gels under denaturizing conditions, and marker proteins were sought through gel densitometry. Proteins became more numerous with insect development. Protein bands were concentrated between 20 and 120 kDa, with the exception of eggs, which presented lower values. The appearance of distinct bands of proteins in the extracts from larvae and adults of G. scutellatus fed on different species of eucalypts reflected changes in their metabolism. These results are useful for integrated pest management, as the identification of marker proteins is possibly associated with metabolic patterns related to the consumption of host plants. Changes in insect diet may affect the marker proteins, indicating a differential ability of G. scutellatus to use host plants.

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          Most cited references29

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          A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding

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            Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

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              Colonization of eucalypts in New Zealand by Australian insects

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                Author and article information

                Journal
                rcen
                Revista Colombiana de Entomología
                Rev. Colomb. Entomol.
                Sociedad Colombiana de Entomología (Bogotá, Distrito Capital, Colombia )
                0120-0488
                2665-4385
                December 2007
                : 33
                : 2
                : 98-103
                Affiliations
                [02] Santiago orgnameUniversidad de Chile orgdiv1Facultad de Ciencias Agronómicas orgdiv2Departamento de Agroindustria y Enología Chile
                [01] Santiago orgnameUniversidad de Chile orgdiv1Facultad de Ciencias Forestales orgdiv2Departamento de Silvicultura Chile ahuerta@ 123456uchile.cl
                Article
                S0120-04882007000200002 S0120-0488(07)03300202
                24595d35-be9b-47ef-b360-fb382bfd5875

                This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

                History
                : 09 September 2006
                : 08 October 2007
                Page count
                Figures: 0, Tables: 0, Equations: 0, References: 31, Pages: 6
                Product

                SciELO Colombia

                Self URI: Texto completo solamente en formato PDF (ES)
                Categories
                Sección agrícola

                Electrophoresis,Eucalypt weevil,Eucalypt snout beetle,Eucalyptus,Gorgojo del eucalipto,Electroforesis

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