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      Hydration and Elevated Calcium Alone Do Not Produce Xylose Nuclear Cataract: Role of Proteolysis by Calpain

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      Ophthalmic Research

      S. Karger AG

      E64, Calpain, Cataract, Xylose, Culture, Calcium

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          The purpose of this experiment was to determine the contribution of calpain proteolytic enzyme (EC in the formation of nuclear cataract during lens culture in xylose. Increased lens calcium was found to be required for formation of xylose nuclear cataract in our culture system. Inhibition of calpain by the cysteine protease inhibitor E64 was effective in slowing the formation of nuclear cataract, even though lens calcium and hydration were markedly elevated. These results showed that hydration and elevated calcium alone do not produce xylose nuclear cataract, and they indicated that calpain proteolysis may be necessary for xylose nuclear cataract in the rat lens.

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          Author and article information

          Ophthalmic Res
          Ophthalmic Research
          S. Karger AG
          10 December 2009
          : 24
          : 1
          : 8-14
          Departments of Biochemistry and Ophthalmology, Schools of Medicine and Dentistry, Oregon Health Sciences University, Portland, Oreg., USA
          267138 Ophthalmic Res 1992;24:8–14
          © 1992 S. Karger AG, Basel

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          Page count
          Pages: 7
          Original Paper

          Vision sciences, Ophthalmology & Optometry, Pathology

          Calcium, E64, Calpain, Cataract, Xylose, Culture


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