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      Assessing the public perceptions of treated wastewater reuse: opportunities and implications for urban communities in developing countries

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          Abstract

          Wastewater reuse has become an integral part of Integrated Water Resources Management and thus plays a role in securing the water needs for future generations. This study aimed at determining the perceptions of Canaanland, an emerging urban community in Ogun State, Nigeria, on treated wastewater reuse for several purposes. Data were collected through questionnaires administered to the city residents (n = 244). Findings revealed that the city was aware of the economic and environmental benefits of wastewater reuse but would prefer reuse schemes that involved less human contact such as flushing toilets, electricity generation, building construction, and car wash. The least preferred option was for potable purposes. The community also revealed that they would be willing to accept wastewater reuse as long as it is endorsed by medical doctors, university professors, and experts. However, 45.5% of the respondents were from the Covenant University academic environment. Also, an assessment was carried out to ascertain the implications and opportunities for wastewater reuse in the city. Findings indicated that wastewater reuse involves several complexities and interlinkages, which revolve around political and decisional factors, economic and social factors, environmental factors, and technological factors. From the study, policy and decisional suggestions and a wastewater process flow were developed for more efficient wastewater management within developing cities. A study was carried out on eight cities from developing nations that have created a framework for wastewater management using several approaches. Also, a summary of findings reveals that if adequately researched, cheap and alternative means of wastewater treatment and reuse could be developed for electricity generation, carwash, and firefighting for developing nations. The result of this research can be used to address public anxieties regarding wastewater-reuse practices. Additionally, this study hopes to aid successful wastewater management schemes in the foreseeable future.

          Abstract

          Environmental science; Public perception; Wastewater; Wastewater reuse; Recycling; Treatment; Urban communities

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          Most cited references114

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          The world’s road to water scarcity: shortage and stress in the 20th century and pathways towards sustainability

          Water scarcity is a rapidly growing concern around the globe, but little is known about how it has developed over time. This study provides a first assessment of continuous sub-national trajectories of blue water consumption, renewable freshwater availability, and water scarcity for the entire 20th century. Water scarcity is analysed using the fundamental concepts of shortage (impacts due to low availability per capita) and stress (impacts due to high consumption relative to availability) which indicate difficulties in satisfying the needs of a population and overuse of resources respectively. While water consumption increased fourfold within the study period, the population under water scarcity increased from 0.24 billion (14% of global population) in the 1900s to 3.8 billion (58%) in the 2000s. Nearly all sub-national trajectories show an increasing trend in water scarcity. The concept of scarcity trajectory archetypes and shapes is introduced to characterize the historical development of water scarcity and suggest measures for alleviating water scarcity and increasing sustainability. Linking the scarcity trajectories to other datasets may help further deepen understanding of how trajectories relate to historical and future drivers, and hence help tackle these evolving challenges.
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            Is Open Access

            Extracellular nanovesicles for packaging of CRISPR-Cas9 protein and sgRNA to induce therapeutic exon skipping

            Prolonged expression of the CRISPR-Cas9 nuclease and gRNA from viral vectors may cause off-target mutagenesis and immunogenicity. Thus, a transient delivery system is needed for therapeutic genome editing applications. Here, we develop an extracellular nanovesicle-based ribonucleoprotein delivery system named NanoMEDIC by utilizing two distinct homing mechanisms. Chemical induced dimerization recruits Cas9 protein into extracellular nanovesicles, and then a viral RNA packaging signal and two self-cleaving riboswitches tether and release sgRNA into nanovesicles. We demonstrate efficient genome editing in various hard-to-transfect cell types, including human induced pluripotent stem (iPS) cells, neurons, and myoblasts. NanoMEDIC also achieves over 90% exon skipping efficiencies in skeletal muscle cells derived from Duchenne muscular dystrophy (DMD) patient iPS cells. Finally, single intramuscular injection of NanoMEDIC induces permanent genomic exon skipping in a luciferase reporter mouse and in mdx mice, indicating its utility for in vivo genome editing therapy of DMD and beyond.
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              The challenges of wastewater irrigation in developing countries

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                Author and article information

                Journal
                Heliyon
                Heliyon
                Heliyon
                The Author(s). Published by Elsevier Ltd.
                2405-8440
                14 October 2020
                October 2020
                14 October 2020
                : 6
                : 10
                : e05246
                Affiliations
                [1]Department of Civil Engineering, College of Engineering, Covenant University, P.M.B., Ota, 112233, Nigeria
                Author notes
                []Corresponding author.
                Article
                S2405-8440(20)32089-2 e05246
                10.1016/j.heliyon.2020.e05246
                7556266
                24e78c9a-76d7-4f9d-874a-895a7c10f349
                © 2020 The Author(s)

                Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.

                History
                : 11 August 2020
                : 13 September 2020
                : 8 October 2020
                Categories
                Research Article

                environmental science,public perception,wastewater,wastewater reuse,recycling,treatment,urban communities

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