Collagen/Polyethylene Oxide Nanofibrous Membranes with Improved Hemostasis and Cytocompatibility for Wound Dressing

Abstract

As a promising agent for biomedical application, collagen has been used as a nanofiber to architecturally mimic its fibrillar structure in Extracellular Matrix (ECM); however, it has to be modified by techniques, such as crosslinking, to overcome its limitations in structural stability along with potential toxicity. Here, we prepared collagen/polyethylene oxide (PEO) nanofibrous membranes with varying crosslinking degrees and their properties, such as water stability, mechanical properties, blood clotting capacity and cytocompatibility, were studied systematically. By investigating the relationship between crosslinking degree and their properties, nanofibrous membranes with improved morphology retention, blood clotting capacity and cytocompatibility have been achieved. The result of circular dichroism measurement demonstrated that a triple helical fraction around 60.5% was retained. Moreover, the electrospun collagen/PEO at crosslinking degrees above 60.6% could maintain more than 72% of its original weight and its nanofibrous morphology under physiological conditions could be well preserved for up to 7 days. Furthermore, the crosslinked collagen/PEO membrane could provide a more friendly and suitable environment to promote cell proliferation, and about 70% of the clot can be formed in 5 min. With its superior performance in water stability, hemostasis and cytocompatibility, we anticipate that this nanofibrous membrane has great potential for wound dressing.