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      Direct analysis of protein complexes using mass spectrometry.

      Nature biotechnology

      Algorithms, Amino Acid Sequence, Chromatography, Liquid, Humans, Mass Spectrometry, methods, Molecular Sequence Data, Ribosomal Proteins, analysis, chemistry, genetics, Ribosomes, Saccharomyces cerevisiae, Sensitivity and Specificity

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          Abstract

          We describe a rapid, sensitive process for comprehensively identifying proteins in macromolecular complexes that uses multidimensional liquid chromatography (LC) and tandem mass spectrometry (MS/MS) to separate and fragment peptides. The SEQUEST algorithm, relying upon translated genomic sequences, infers amino acid sequences from the fragment ions. The method was applied to the Saccharomyces cerevisiae ribosome leading to the identification of a novel protein component of the yeast and human 40S subunit. By offering the ability to identify >100 proteins in a single run, this process enables components in even the largest macromolecular complexes to be analyzed comprehensively.

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          Journal
          10404161
          10.1038/10890

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