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      Direct analysis of protein complexes using mass spectrometry.

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          Abstract

          We describe a rapid, sensitive process for comprehensively identifying proteins in macromolecular complexes that uses multidimensional liquid chromatography (LC) and tandem mass spectrometry (MS/MS) to separate and fragment peptides. The SEQUEST algorithm, relying upon translated genomic sequences, infers amino acid sequences from the fragment ions. The method was applied to the Saccharomyces cerevisiae ribosome leading to the identification of a novel protein component of the yeast and human 40S subunit. By offering the ability to identify >100 proteins in a single run, this process enables components in even the largest macromolecular complexes to be analyzed comprehensively.

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          Author and article information

          Journal
          Nat Biotechnol
          Nature biotechnology
          Springer Science and Business Media LLC
          1087-0156
          1087-0156
          Jul 1999
          : 17
          : 7
          Affiliations
          [1 ] Department of Molecular Biotechnology, University of Washington, Seattle 98195, USA.
          Article
          10.1038/10890
          10404161
          2535a76a-d3ad-4e06-a9ad-6bc7914451c2
          History

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