High Resolution Melting-Typing (HRMT) of methicillin-resistant Staphylococcus aureus (MRSA): The new frontier to replace multi-locus sequence typing (MLST) for epidemiological surveillance studies.

Mongelli, Gino; Bongiorno, Dafne; Agosta, Marilena; Benvenuto, Sabrina; Stefani, Stefania; Campanile, Floriana

doi:10.1016/j.mimet.2015.08.001

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High Resolution Melting-Typing (HRMT) of methicillin-resistant Staphylococcus aureus (MRSA): The new frontier to replace multi-locus sequence typing (MLST) for epidemiological surveillance studies.

Author(s): Gino Mongelli ¹ , Dafne Bongiorno ¹ , Marilena Agosta ¹ , Sabrina Benvenuto ¹ , Stefania Stefani ¹ , Floriana Campanile ²

Publication date (Electronic): Oct 2015

Journal: Journal of microbiological methods

Publisher: Elsevier BV

Keywords: Cost and time reduction, High Resolution Melting Typing (HRMT), Methicillin-resistant Staphylococcus aureus (MRSA), Molecular-epidemiology, Single-nucleotide polymorphisms (SNPs)

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Abstract

We report an implemented molecular-typing-method based on HRMA to detect SNPs within MLST loci, characterizing 100 clinical MRSA and 11 control strains, representative of Italian clones. The results provide solid evidence that HRMT could be a fast, cost-effective and reliable alternative to MLST, for MRSA molecular epidemiology.

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Journal ID (iso-abbrev): J. Microbiol. Methods

Title: Journal of microbiological methods

Publisher: Elsevier BV

ISSN (Electronic): 1872-8359

ISSN (Print): 0167-7012

Publication date (Electronic): Oct 2015

Volume: 117

Affiliations

[1 ] Medical Molecular Microbiology and Antibiotic Resistance laboratory (MMAR Lab), Department of Biomedical and Biotechnological Sciences, University of Catania, Italy.

[2 ] Medical Molecular Microbiology and Antibiotic Resistance laboratory (MMAR Lab), Department of Biomedical and Biotechnological Sciences, University of Catania, Italy. Electronic address: f.campanile@unict.it.

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Publisher Item ID: S0167-7012(15)30034-8

DOI: 10.1016/j.mimet.2015.08.001

PubMed ID: 26255133

SO-VID: 2587a0b9-5078-44ed-a89d-44c0ad468f8a

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Keywords: Cost and time reduction,High Resolution Melting Typing (HRMT),Methicillin-resistant Staphylococcus aureus (MRSA),Molecular-epidemiology,Single-nucleotide polymorphisms (SNPs)

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Keywords: Cost and time reduction, High Resolution Melting Typing (HRMT), Methicillin-resistant Staphylococcus aureus (MRSA), Molecular-epidemiology, Single-nucleotide polymorphisms (SNPs)

High Resolution Melting-Typing (HRMT) of methicillin-resistant Staphylococcus aureus (MRSA): The new frontier to replace multi-locus sequence typing (MLST) for epidemiological surveillance studies.

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