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      Simultaneous multi-lifetime multi-color STED imaging for colocalization analyses.

      Optics Express
      Animals, Cell Line, Color, Imaging, Three-Dimensional, methods, Lamins, metabolism, Microscopy, Fluorescence, Time Factors, Tubulin

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          Abstract

          We describe a STED microscope optimized for colocalization experiments with up to three colors. Two fluorescence labels are separated by their fluorescence lifetime whereas a third channel is discriminated by the wavelength of fluorescence emission. Since it does not require a second STED beam, separating by lifetime is insensitive to drift and thus optimally suited for colocalization analyses. Furthermore, we propose a setup having a second STED beam for long duration multicolor recording.

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          Author and article information

          Journal
          21369135
          10.1364/OE.19.003130

          Chemistry
          Animals,Cell Line,Color,Imaging, Three-Dimensional,methods,Lamins,metabolism,Microscopy, Fluorescence,Time Factors,Tubulin

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