Due to a mistake in data set export, an error was introduced by which CTD-2328D6.1
was mistakenly switched to RNA28S5 in Table S5, leading to this gene wrongly being
mentioned in the text on several occasions, and errors in Fig. 4.
The corrected Table S5 is shown in the supplementary material.
On page 2046, section‚ ‘Host response-associated genes, including type III IFNs, are
activated upon TBEV infection of DAOY cells’, the first appearance of ‘RNA28S5’ should
be changed to ‘CTD-2328D6.1’ and the final appearance of ‘RNA28S5’ should be removed
from the following section of text.
‘RNA28S5, RN7SL2, NOTCH3, COL1A1, BCL9L, BCORL1, POLR2A, FAM71D, IGF2, RN7SL3 and
HSPG2 were found to be the most strongly down-regulated genes (fold-change >2.5; Fig.
4 and Table S5). Other than protein-coding genes, a number of non-coding RNAs were
also identified as being differentially expressed upon TBEV infection, as shown in
Fig. 4(b). However, of these, RN7SL2, RN7SL3 and RNA28S5 are the only RNA genes with
known functions.’
This section should read as follows:
‘CTD-2328D6.1, RN7SL2, NOTCH3, COL1A1, BCL9L, BCORL1, POLR2A, FAM71D, IGF2, RN7SL3
and HSPG2 were found to be the most strongly down-regulated genes (fold-change >2.5;
Fig. 4 and Table S5). Other than protein-coding genes, a number of non-coding RNAs
were also identified as being differentially expressed upon TBEV infection, as shown
in Fig. 4(b). However, of these, RN7SL2 and RN7SL3 are the only RNA genes with known
functions.’
On page 2052, right-hand column, paragraph 2, the first occurrence of ‘RNA28S5’ should
be removed and the final appearance of ‘RNA28S5’ should be changed to ‘CTD-2328D6.1’
in the following section of text.
‘Downregulation of effectors involved in either transcription (POLR2A) or translation
(RNA28S5, RN7SL2, RN7SL3) suggests a possible TBEV-driven transcriptional or translational
shut off in host cells. Both transcriptional and translational shut off are well-documented
phenomena [63, 64]. Similar rates of RNA28S5, NOTCH3, COL1A1, BCL9L, BCOR1, POLR2A,
FAM71D, IGF2 and HSPG2 down-regulation were also evident in IFN-β-pre-treated cells
infected with TBEV, where significantly lower viral titres were determined.’
This section should read as follows:
‘Downregulation of effectors involved in either transcription (POLR2A) or translation
(RN7SL2, RN7SL3) suggests a possible TBEV-driven transcriptional or translational
shut off in host cells. Both transcriptional and translational shut off are well-documented
phenomena [63, 64]. Similar rates of CTD-2328D6.1, NOTCH3, COL1A1, BCL9L, BCOR1, POLR2A,
FAM71D, IGF2 and HSPG2 down-regulation were also evident in IFN-β-pre-treated cells
infected with TBEV, where significantly lower viral titres were determined.’
An error also occured in Table S3 due to a formatting mistake. The following changes
are shown in the updated supplementary material:
‘3.01’ on page 1 in column ‘IFN-β’ was corrected to ‘MARC1’.
‘9.09’ on page 10 in column ‘TBEV’ was corrected to ‘SEPT9’.
‘3.04’ on page 12 in column ‘TBEV’ was corrected to ‘MARCH4’.
‘9.09’ on page 14 in column ‘IFN-β + TBEV’ was corrected to ‘SEPT9’.
‘3.03’ on page 20 in column ‘IFN-β + TBEV’ was corrected to ‘MARCH3’.
Fig. 4 also required a correction in the heatmap and four genes that were in panel
(a) should only be present in panel (b), with RNA28S5 deleted from the figure. The
corrected Fig. 4 is shown above.
Fig. 4.
Overview of selected differentially expressed genes. DAOY cells were pre-treated with
IFN-β (10 ng ml−1) and/or infected with TBEV (m.o.i. 5) after 12 h. Three independent
biological replicates were included for each of the combinations [untreated mock cells
(control); IFN-β-treated mock cells; untreated cells infected with TBEV; IFN-β-pre-treated
cells infected with TBEV]. Total cellular RNA was isolated at 24 h p.i. and used for
transcriptome analysis. (a) List of selected protein-coding genes identified to be
differentially expressed in at least one of the combinations over control (Benjamini
Hochberg P-value ≤0.05 and fold change >1.5 or <−1.5; down-regulated in red and up-regulated
in green). To emphasize the up-regulation of IFN-λ1, information for transcripts of
IFN-ɑ, IFN-β and IFN-γ was also included. (b) List of selected non-coding genes identified
to be differentially expressed in at least one of the combinations over control (Benjamini
Hochberg P-value ≤0.05 and fold change >1.5 or <−1.5; down-regulated in red and up-regulated
in green).
The authors apologise for any inconvenience caused.