Zhenbo Han 1 , 2 , Zihang Xu 1 , 2 , Lei Chen 3 , Danyu Ye 1 , 2 , Yang Yu 1 , 2 , Ying Zhang 1 , 2 , Yang Cao 1 , 2 , Bamba Djibril 1 , 2 , Xiaofei Guo 1 , 2 , Xinlu Gao 1 , 2 , Wenwen Zhang 1 , 2 , Meixi Yu 1 , 2 , Shenzhen Liu 1 , 2 , Gege Yan 1 , 2 , Mengyu Jin 1 , 2 , Qi Huang 1 , 2 , Xiuxiu Wang 1 , 2 , Bingjie Hua 1 , 2 , Chao Feng 1 , 2 , Fan Yang 1 , 2 , Wenya Ma , 1 , 2 , Yu Liu , 4
07 April 2020
Iron overload affects the cell cycle of various cell types, but the effect of iron overload on human pluripotent stem cells has not yet been reported. Here, we show that the proliferation capacities of human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs) were significantly inhibited by ferric ammonium citrate (FAC) in a concentration‐dependent manner. In addition, deferoxamine protected hESCs/hiPSCs against FAC‐induced cell‐cycle arrest. However, iron overload did not affect pluripotency in hESCs/hiPSCs. Further, treatment of hiPSCs with FAC resulted in excess reactive oxygen species production and DNA damage. Collectively, our findings provide new insights into the role of iron homeostasis in the maintenance of self‐renewal in human pluripotent stem cells.
We demonstrated in this study that iron overload could severely impair the proliferation of human embryonic stem cells/human induced pluripotent stem cells through excess reactive oxygen species production and DNA damage. These results imply a role of iron homeostasis in maintaining the self‐renewal of human embryonic stem cells/human induced pluripotent stem cells.