There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.
Abstract
Double-stranded RNA representing four distinct electrophoretic patterns was found
in a screen of Phytophthora infestans isolates. Two dsRNAs that always appeared together
were sequenced. RNA 1, which was 3160 nt plus a poly (A) tail, contained a single
deduced ORF with the potential to encode a polyprotein of 977 aa with motifs characteristic
of supergroup I viral RdRps. The 2776 nt, polyadenylated RNA2 contained an ORF with
a potential to encode a polyprotein of 847 aa including a possible trypsin-like serine
protease, and a second putative ORF of unknown function. An alternative form of RNA2,
in which a 19-nt stretch was replaced by a 9-nt sequence, was detected in 4 of 17
clones sequenced. Based on genome structure and phylogenetic analysis, this virus
did not fit into any known virus family and we tentatively named it Phytophthora infestans
RNA virus 1 (PiRV-1).