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      Isolation and Expression Analysis of Growth-Related Genes at Different Growth Stages of Dinoflagellate Alexandrium pacificum

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          Abstract

          Based on the transcriptomic sequencing, three genes of Alexandrium pacificum encoding ubiquitin ( UBI), telomerase ( TEL) and glycine-rich protein ( GRP) relating to cell division were isolated and characterized. The full-length cDNA of GRP was obtained through approach of rapid amplification of cDNA ends (RACE). Four conserved domains, including DNA- and RNA- binding sites or motifs, cold shock domain at the N-terminal, and zinc-finger structure of CCCH type at the C-terminal were idenrtified. Phylogenetic analysis revealed that the deduced amino acid sequence of GRP tends to cluster together with proteins harboring cold shock domain. The expressions of these three genes were analyzed with quantitative PCR (qPCR). It was found that the expressions of these three genes at the logarithmic growth phase and induced logarithmic growth phase were all higher than those at lagging growth phase ( P < 0.05). The expression patterns of these three genes were coincident with the proliferative capacity of the algae, e.g., displaying increased expression level at log and induced growth phases. The functions of these genes and their possible roles during harmful algal blooms (HAB) were discussed.

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          Author and article information

          Journal
          JOUC
          Journal of Ocean University of China
          Science Press and Springer (China )
          1672-5182
          14 May 2019
          01 June 2019
          : 18
          : 3
          : 710-718
          Affiliations
          1 Key Laboratory of Marine Genetics and Breeding of Ministry of Education, Ocean University of China, Qingdao 266003, China
          Author notes
          *Corresponding author: SUI Zhenghong
          Article
          s11802-019-3849-y
          10.1007/s11802-019-3849-y
          Copyright © Ocean University of China, Science Press and Springer-Verlag GmbH Germany 2019.

          The copyright to this article, including any graphic elements therein (e.g. illustrations, charts, moving images), is hereby assigned for good and valuable consideration to the editorial office of Journal of Ocean University of China, Science Press and Springer effective if and when the article is accepted for publication and to the extent assignable if assignability is restricted for by applicable law or regulations (e.g. for U.S. government or crown employees).

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          Self URI (journal-page): https://www.springer.com/journal/11802

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