Blog
About

257
views
0
recommends
+1 Recommend
0 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: not found

      Optical sectioning deep inside live embryos by selective plane illumination microscopy.

      Science (New York, N.Y.)

      Oryzias, embryology, Drosophila melanogaster, Embryo, Nonmammalian, Animals, Genetically Modified, anatomy & histology, cytology, Embryonic Development, Green Fluorescent Proteins, Heart, Image Processing, Computer-Assisted, Imaging, Three-Dimensional, Lasers, Light, Luminescent Proteins, analysis, Microscopy, Fluorescence, methods, Morphogenesis, Anatomy, Cross-Sectional, Animals

      Read this article at

      ScienceOpenPublisherPubMed
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          Large, living biological specimens present challenges to existing optical imaging techniques because of their absorptive and scattering properties. We developed selective plane illumination microscopy (SPIM) to generate multidimensional images of samples up to a few millimeters in size. The system combines two-dimensional illumination with orthogonal camera-based detection to achieve high-resolution, optically sectioned imaging throughout the sample, with minimal photodamage and at speeds capable of capturing transient biological phenomena. We used SPIM to visualize all muscles in vivo in the transgenic Medaka line Arnie, which expresses green fluorescent protein in muscle tissue. We also demonstrate that SPIM can be applied to visualize the embryogenesis of the relatively opaque Drosophila melanogaster in vivo.

          Related collections

          Author and article information

          Journal
          15310904
          10.1126/science.1100035

          Comments

          Comment on this article