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      Cross-linked enzyme aggregates: a simple and effective method for the immobilization of penicillin acylase.

      Organic Letters

      Ampicillin, chemical synthesis, Cross-Linking Reagents, Enzymes, Immobilized, metabolism, Solvents, Hydrolysis, Kinetics, Penicillin Amidase

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          Abstract

          [reaction--see text] Penicillin G acylase (penicillin amidohydrolase, E.C. 3.5.1.11) was immobilized in a simple and effective way by physical aggregation of the enzyme, using a precipitant, followed by chemical cross-linking to form insoluble cross-linked enzyme aggregates (CLEAs). These had the same activity in the synthesis of ampicillin as cross-linked crystals of the same enzyme, but the accompanying hydrolysis of the side-chain donor was much less. Penicillin G acylase CLEAs also catalyzed the synthesis of ampicillin in a broad range of organic solvents.

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          10814447

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