Wolf spider (Araneae: Lycosidae) assemblages were studied in a deciduous forest from a Natura 2000 site in north-western Romania. A number of 1661 adults belonging to 19 species were collected by pitfall trapping. The highest abundance was achieved by Pardosa alacris in all of the three analyzed sites (marsh, forest edge, forest interior), followed by Trochosa spinipalpis in the marsh and forest, Trochosa terricola in the edge and forest, Alopecosa pulverulenta, Piratula hygrophila in the marsh and Arctosa lutetiana in the edge. Both the observed and estimated species richness was greatest in the marsh, while the inventory completeness was highest in the edge. The presence of some rare species ( Hygrolycosa rubrofasciata, Pardosa maisa) along with the different composition of the wolf spider communities highlight the need of conserving the original (micro)habitat heterogeneity of natural forests for ensure the survival of diverse ground-dwelling spider assemblages.