17
views
0
recommends
+1 Recommend
1 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: found

      Global identification of chemical constituents and rat metabolites of Si-Miao-Wan by liquid chromatography-electrospray ionization/quadrupole time-of-flight mass spectrometry

      Read this article at

      ScienceOpenPublisher
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          Si-Miao-Wan (SMW), a tradiational Chinese medicinal formula consisting of Atractylodis Rhizoma, Phellodendri Chinensis Cortex, Coicis Semen, and Achyranthis Bidentatae Radix, has been used for the treatment of gout and gouty arthritis for many years. In the present study, a liquid chromatography quadrupole-time-of-flight mass spectrometry (LC-Q-TOF/MS) method was established to identify the multiple constituents of SMW and its metabolites in rat biological samples after oral administration. A total of 48 compounds in SMW, including 21 alkaloids, 12 organic acids, 2 terpenes, 3 lactones, 2 phytosterols, and 8 other compounds, were tentatively characterized with the diagnostic-ion filtering strategy. Based on the diagnostic ions applied to identify compounds in SMW, 28 prototype compounds and 10 metabolic compounds were detected in the biological samples. This was the first comprehensive drug metabolism investigation of SMW in rats. The developed method could be a useful means for identifying the multi-components in SMW and the metabolic components. The results may help explore the possible metabolic processes and mechanism of action for SMW in vivo.

          Related collections

          Author and article information

          Journal
          CJNM
          Chinese Journal of Natural Medicines
          Elsevier
          1875-5364
          20 July 2017
          : 15
          : 7
          : 550-560
          Affiliations
          1State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, China
          Author notes
          *Corresponding author: CHEN Jun, Tel: 86-25-83271382, Fax: 86-25-83271379, E-mail: chenj2002cpu@ 123456126.com

          These authors have no conflict of interest to declare.

          Article
          S1875-5364(17)30082-1
          10.1016/S1875-5364(17)30082-1
          Copyright © 2017 China Pharmaceutical University. Published by Elsevier B.V. All rights reserved.
          Funding
          Funded by: National Natural Science Foundation of China
          Award ID: 81573705
          Funded by: Natural Science Foundation of Jiangsu Province
          Award ID: BK20171392
          This work was supported by the National Natural Science Foundation of China (No. 81573705), the Natural Science Foundation of Jiangsu Province (No. BK20171392) and the Priority Academic Program Development of Jiangsu Higher Education Institutions.

          Comments

          Comment on this article