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      The Effects of the NMR Shift-reagents Dy(PPP)2, Dy(TTHA) and Tm(DOTP) on Developed Pressure in Isolated Perfused Rat Hearts. The Role of Shift-reagent Calcium Complexes

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      Journal of Molecular and Cellular Cardiology

      Elsevier BV

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          Influence of global ischemia on intracellular sodium in the perfused rat heart.

          Intracellular [Na+], [H+], and [ATP] and mechanical performance were measured in the isovolumic perfused rat heart during ischemia. The concentration of intracellular sodium, [Na+]i, was determined by atomic absorption spectroscopy under control conditions, and [Na+]i was monitored by 23Na NMR spectroscopy at 1-min intervals under control conditions and during global ischemia. [ATP], [H+], and [Pi] were measured by 31P NMR in a separate group under identical conditions. The control [Na+]i measured by atomic absorption was 30.7 +/- 3.3 mM (mean +/- SD, n = 6), and [Na+]i measured by NMR was 6.2 +/- 0.5 mM (n = 3). Brief ischemia (10 min) was associated with a 54% increase in [Na+]i which reversed completely with reperfusion. Developed pressure also returned to control values upon reperfusion. Prolonged ischemia (30 min) produced continuous further accumulation of sodium (0.53 mM/min, r2 = 0.99). [H+] also increased approximately linearly early in ischemia (0.084 microM/min, r2 = 0.97). The rate of increase in [Na+]i was more than 4000 times greater than the increase in [H+] on a molar basis. Nevertheless, [H+]/[Na+]i increased early in ischemia because the proportional change in [H+] was greater than that in [Na+]i. These results indicate that (1) intracellular sodium measured by NMR in the functioning heart is about 20% of total intracellular sodium; (2) intracellular acidosis and accumulation of sodium develop simultaneously during global ischemia; (3) increased intracellular sodium content is not in itself an indicator of irreversible injury; and (4) recovery of mechanical performance is associated with return of [Na+]i (measured by NMR) to baseline after brief ischemia. The mechanism of the increase in sodium content detected by NMR is unknown.
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            Tm(DOTP)5−: A23Na+ shift agent for perfused rat hearts

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              23Na and 39K nuclear magnetic resonance studies of perfused rat hearts. Discrimination of intra- and extracellular ions using a shift reagent

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                Author and article information

                Journal
                Journal of Molecular and Cellular Cardiology
                Journal of Molecular and Cellular Cardiology
                Elsevier BV
                00222828
                November 2001
                November 2001
                : 33
                : 11
                : 1945-1956
                Article
                10.1006/jmcc.2001.1459
                © 2001

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