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      Effects of dopamine on proliferation, invasion, and expression of vascular endothelial growth factor in glioma cell line U251

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          Abstract

          Objective To explore effects and significances of dopamine on the proliferation, invasion, and expression of vascular endothelial growth factor (VEGF) in glioma cell line U251.

          Methods Human glioma cells line U251 were divided into 5 groups administered with dopamine at doses of 0 (control), 10, 25, 50, and 100 μmol/L. CCK-8 method was used to detect cell proliferation at 0, 12, 24, 36 and 48 hours of dopamine treatment. Flow cytometry and Transwell assay were used to detect changes of cell cycle and invasive ability 48 hours after dopamine treatment. The expressions of cyclin D1, cyclin dependent kinase 4 (CDK-4), cyclin dependent kinase 6 (CDK-6), VEGF, matrix metalloproteinase 2 (MMP-2), and matrix metalloproteinase 9 (MMP-9) were detected with Western blot.

          Results Compared to the control cells, the U251 cells with 48 hours′ treatment of dopamine at doses of 10, 25, 50, and 100 μmol/L showed following significant variations in significant dose-dependent manners: decreased cell proliferation percentage (46.82 ± 1.17%, 41.96 ± 1.06%, 37.52 ± 0.98%, and 30.57 ± 1.11% vs. 92.36 ± 1.37%, P < 0.05 for all); increased percentage of cells in G0/G1 phase ( P < 0.05 for all); decreased percentage of cells in S phase ( P < 0.05 for all); reduced number of invasive cells (776.52 ± 25.16, 555.43 ± 22.57, 442.33 ± 12.03, and 336.82 ± 14.11 vs. 1 099.82 ± 33.57, P < 0.05 for all); and decreased intracellular expressions of cyclin D1, CDK4, CDK6, VEGF, MMP-2, and MMP-9 (all P < 0.05).

          Conclusion Dopamine could inhibit the proliferation and invasion of glioma cell line U251 and the effects may be related to the inhibited intracellular expressions of cyclin D1, CDK-4, CDK-6, VEGF, MMP-2, and MMP-9.

          Abstract

          【摘 要】 目的 探讨多巴胺对胶质瘤细胞 U251 增殖、侵袭及血管内皮生长因子(VEGF)表达的影响及意义。 方法 将人胶质瘤细胞株 U251 分为 5 组,分别加入多巴胺 0(对照)、10、25、50、100 μmol/L,分别于培养 12、24、36、48 h 时,采用细胞计数试剂盒(CCK-8)法检测细胞增殖情况,采用流式细胞仪和 Transwell 实验检测培养 48 h 后细胞周期及侵袭能力变化,采用 Western blot 检测细胞中细胞周期蛋白 D1(cyclin D1)、细胞周期蛋白依赖性激酶 4(CDK-4)、 CDK-6 及 VEGF、基质金属蛋白酶 2(MMP-2)、MMP-9 表达。 结果 与对照组[(92.36 ± 1.37)%]比较,多巴胺培养 48 h 时,10、25、50 和 100 μmol/L 组 U251 细胞增殖百分比[(46.82 ± 1.17)%、(41.96 ± 1.06)%、(37.52 ± 0.98)%、(30.57 ± 1.11)%]明显下降( P < 0.05),呈剂量依赖性( P < 0.05);与对照组比较,多巴胺 10、25、50 和 100 μmol/L 组 U251 细胞 G0/G1 期比例明显增多( P < 0.05),S 期细胞比例明显减少( P < 0.05),具有剂量依赖性;与对照组[(1 099.82 ± 33.57)个] 比较,多巴胺 10、25、50 和 100 μmol/L 组 U251 细胞侵袭数目[分别为(776.52 ± 25.16)、(555.43 ± 22.57)、(442.33 ± 12.03)、(336.82 ± 14.11)个]均明显减少( P < 0.05),呈一定剂量依赖性( P < 0.05);与对照组比较,多巴胺 10、25、50 和 100 μmol/L 组 U251 细胞中 cyclin D1、CDK4、CDK6 及 VEGF、MMP-2、MMP-9 蛋白表达量均明显降低( P < 0.05)。

          Author and article information

          Journal
          CPH
          Chinese Journal of Public Health
          Chinese Journal of Public Health (China )
          1001-0580
          01 May 2019
          01 March 2019
          : 35
          : 5
          : 563-566
          Affiliations
          [1] 1Department of Neurosurgery, International Hospital of Peking University, Beijing 102226, China
          Article
          zgggws1122471
          10.11847/zgggws1122471
          2789ac9d-56bf-42d1-8d5f-2fe90490545b
          © 2019 China Public Health Editorial Department

          This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 Unported License (CC BY-NC 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. See https://creativecommons.org/licenses/by-nc/4.0/.

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          Categories
          Journal Article

          Medicine,Nutrition & Dietetics,Occupational & Environmental medicine,Health & Social care,Infectious disease & Microbiology,Public health
          invasion,dopamine,vascular endothelial growth factor,glioma cell line U251,proliferation

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