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      DNA barcoding of euryglossine bees and the description of new species of Euhesma Michener (Hymenoptera, Colletidae, Euryglossinae)

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          Abstract

          Abstract

          This paper launches an open access DNA barcoding project “AUSBS” under the [

          Barcoding of Life Datasystems

          ](BOLD). The aims of the project are to help scientists who lack the necessary morphological knowledge to identify known species using molecular markers, to aid native bee specialists with the recognition of species groups that morphologically are difficult to define, and, eventually, to assist with the recognition of new species among known species. Using integrative taxonomy, i.e. morphological comparison to type specimens in Australian museum collections combined with phylogenetic analysis of a fragment of the [

          mitochondrial DNA cytochrome c oxidase subunit I

          ](mtCOI) gene sequences led to the recognition of four new species of Euhesma Michener ( Hymenoptera : Colletidae : Euryglossini ) collected during intensive surveys in remote Australian conservation areas, which are described. The new species are Euhesma micans , Euhesma lyngouriae , and Euhesma aulaca in a species group associated with Eremophila flowers, and Euhesma albamala in the walkeriana species group.

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          Little left to lose: deforestation and forest degradation in Australia since European colonization

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            DNA barcoding largely supports 250 years of classical taxonomy: identifications for Central European bees (Hymenoptera, Apoidea partim).

            This study presents DNA barcode records for 4118 specimens representing 561 species of bees belonging to the six families of Apoidea (Andrenidae, Apidae, Colletidae, Halictidae, Megachilidae and Melittidae) found in Central Europe. These records provide fully compliant barcode sequences for 503 of the 571 bee species in the German fauna and partial sequences for 43 more. The barcode results are largely congruent with traditional taxonomy as only five closely allied pairs of species could not be discriminated by barcodes. As well, 90% of the species possessed sufficiently deep sequence divergence to be assigned to a different Barcode Index Number (BIN). In fact, 56 species (11%) were assigned to two or more BINs reflecting the high levels of intraspecific divergence among their component specimens. Fifty other species (9.7%) shared the same Barcode Index Number with one or more species, but most of these species belonged to a distinct barcode cluster within a particular BIN. The barcode data contributed to clarifying the status of nearly half the examined taxonomically problematic species of bees in the German fauna. Based on these results, the role of DNA barcoding as a tool for current and future taxonomic work is discussed.
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              DNA barcoding and the mediocrity of morphology.

              A small but vocal community of critics has questioned the epistemological value of DNA barcoding by suggesting that either it 'cannot work' for the identification or discovery of species or that it ignores the 'richness' inherent in traditional approaches. We re-examine these arguments through a comparison of DNA barcoding and morphological taxonomy in terms of their accuracy and diversity of characters employed. We conclude that morphology often does not work and that it is often nowhere near as 'rich' as has been argued. Morphology is particularly poor in numerous important situations, such as the association of larvae with adults and discrimination among cryptic species. The vehemence of some of the criticisms is surprising given that morphology alone is known to be inadequate to the task of species-level identification in many instances. © 2009 Blackwell Publishing Ltd.
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                Author and article information

                Journal
                Zookeys
                Zookeys
                ZooKeys
                ZooKeys
                Pensoft Publishers
                1313-2989
                1313-2970
                2015
                16 September 2015
                : 520
                : 41-59
                Affiliations
                [1 ]School of Agriculture, Food and Wine, The University of Adelaide, SA 5005
                [2 ]South Australian Museum, North Terrace, Adelaide, SA 5000
                [3 ]School of Pharmacy and Medical Sciences, University of South Australia, Adelaide, SA 5000
                Author notes
                Corresponding author: Katja Hogendoorn ( katja.hogendoorn@ 123456adelaide.edu.au )

                Academic editor: M. Ohl

                Article
                10.3897/zookeys.520.6185
                4591721
                26448713
                27a63cae-1968-47d4-a6fa-6b52d58c6577
                Katja Hogendoorn, Mark Stevens, Remko Leijs

                This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

                History
                : 13 February 2015
                : 24 August 2015
                Categories
                Research Article

                Animal science & Zoology
                barcoding,species discovery,euryglossine bees,bush blitz survey,conservation,animalia,hymenoptera,colletidae

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