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Telomere measurement by quantitative PCR
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Abstract
It has long been presumed impossible to measure telomeres in vertebrate DNA by PCR
amplification with oligonucleotide primers designed to hybridize to the TTAGGG and
CCCTAA repeats, because only primer dimer-derived products are expected. Here we present
a primer pair that eliminates this problem, allowing simple and rapid measurement
of telomeres in a closed tube, fluorescence-based assay. This assay will facilitate
investigations of the biology of telomeres and the roles they play in the molecular
pathophysiology of diseases and aging.