Blog
About

116
views
0
recommends
+1 Recommend
0 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: not found

      Multiple nutrient stresses at intersecting Pacific Ocean biomes detected by protein biomarkers.

      Science (New York, N.Y.)

      Stress, Physiological, Biological Markers, microbiology, Seawater, methods, Proteomics, metabolism, Proteins, Prochlorococcus, Phosphorus, Pacific Ocean, Nitrogen, Iron, Cobalt

      Read this article at

      ScienceOpenPublisherPubMed
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          Marine primary productivity is strongly influenced by the scarcity of required nutrients, yet our understanding of these nutrient limitations is informed by experimental observations with sparse geographical coverage and methodological limitations. We developed a quantitative proteomic method to directly assess nutrient stress in high-light ecotypes of the abundant cyanobacterium Prochlorococcus across a meridional transect in the central Pacific Ocean. Multiple peptide biomarkers detected widespread and overlapping regions of nutritional stress for nitrogen and phosphorus in the North Pacific Subtropical Gyre and iron in the equatorial Pacific. Quantitative protein analyses demonstrated simultaneous stress for these nutrients at biome interfaces. This application of proteomic biomarkers to diagnose ocean metabolism demonstrated Prochlorococcus actively and simultaneously deploying multiple biochemical strategies for low-nutrient conditions in the oceans. Copyright © 2014, American Association for the Advancement of Science.

          Related collections

          Most cited references 57

          • Record: found
          • Abstract: found
          • Article: not found

          Community genomics among stratified microbial assemblages in the ocean's interior.

          Microbial life predominates in the ocean, yet little is known about its genomic variability, especially along the depth continuum. We report here genomic analyses of planktonic microbial communities in the North Pacific Subtropical Gyre, from the ocean's surface to near-sea floor depths. Sequence variation in microbial community genes reflected vertical zonation of taxonomic groups, functional gene repertoires, and metabolic potential. The distributional patterns of microbial genes suggested depth-variable community trends in carbon and energy metabolism, attachment and motility, gene mobility, and host-viral interactions. Comparative genomic analyses of stratified microbial communities have the potential to provide significant insight into higher-order community organization and dynamics.
            Bookmark
            • Record: found
            • Abstract: not found
            • Article: not found

            Processes and patterns of oceanic nutrient limitation

             C Moore,  M. Mills,  K. Arrigo (2013)
              Bookmark
              • Record: found
              • Abstract: not found
              • Article: not found

              Empirical Statistical Model To Estimate the Accuracy of Peptide Identifications Made by MS/MS and Database Search

                Bookmark

                Author and article information

                Journal
                10.1126/science.1256450
                25190794

                Comments

                Comment on this article