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      Ascorbic acid transport and distribution in human B lymphocytes.

      Archives of Biochemistry and Biophysics
      Ascorbic Acid, metabolism, B-Lymphocytes, Biological Transport, Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone, pharmacology, Cell Compartmentation, Cells, Cultured, Culture Media, Humans, Ouabain, Palatine Tonsil

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          Ascorbic acid (vitamin C) transport was investigated in human B lymphocytes. The vitamin was transported by two components. The first was a high-affinity activity with an apparent Km of 7-10 microM and Vmax of 0.14 mM/h (3.11 x 10(-4) mumol x h-1 x mg protein-1). The activity was concentration and temperature dependent, saturable, and inhibited by carbonylcyanide-p-trifluoromethoxyphenylhydrazone and ouabain and generated ascorbic acid accumulation against a concentration gradient. Kinetics for the second component were indeterminate because ascorbate was not accumulated against a concentration gradient. Subcellular fractionation revealed that intracellular ascorbic acid in human B lymphocytes was > 90% localized to the cytosol and not protein bound. Kinetic parameters of high-affinity ascorbic acid transport could operate effectively with plasma concentrations normally found in humans.

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