10
views
0
recommends
+1 Recommend
0 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: found
      Is Open Access

      Morphometry of the basal cell layer of oral leukoplakia and oral squamous cell carcinoma using computer-aided image analysis

      research-article

      Read this article at

      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          Objectives:

          To study and compare the changes in nuclear and cellular size, shape and nuclear–cytoplasmic ratio of the cells in the basal layer of oral leukoplakia and well-differentiated oral squamous cell carcinoma (SCC) with normal buccal mucosa, using computer-aided image analysis in tissue sections.

          Study design:

          This was a retrospective study conducted on tissue sections on a total number of 70 cases to determine the various morphometric parameters. The data collected in this study were analyzed statistically by computing descriptive statistics, viz., percentage, mean, standard deviation, standard error of mean, 95% confidence interval for mean. The difference in the control and study groups for various diagnostic variables was compared by means of analysis of variance (ANOVA), Student’s t-test for independent samples, wherever applicable. Mann–Whitney U-test and Kruskal–Wallis test were used where the data were found to be asymmetrical and the standard deviations were also different. The results were considered statistically significant whenever P ≤ 0.05.

          Results:

          Our results were significant for the morphometric parameter, size. The values of nuclear perimeter and area, cellular perimeter and area increased gradually from the normal buccal mucosa to leukoplakia, reaching the highest value in SCC. There was statistically significant difference in the nuclear and cellular areas to differentiate between leukoplakia and squamous cell carcinoma. Two variables which were used to study the shape, “form perimeter (PE)” and “contour index (CI)”, showed significant difference between normal buccal mucosa and leukoplakia and between normal buccal mucosa and SCC. The morphometric parameter, nuclear–cytoplasmic ratio, in our results showed an increase in leukoplakia and SCC compared to normal buccal mucosa, but the difference was not significant between leukoplakia and SCC.

          Conclusion:

          The morphometric parameter, size, was useful to differentiate between normal, potentially malignant leukoplakia and SCC.

          Related collections

          Most cited references33

          • Record: found
          • Abstract: found
          • Article: not found

          The epidemiology of mouth cancer: a review of global incidence.

          Mouth cancer (143-145 ICD-9) is a major health problem in many parts of the world. While its incidence is relatively low in most western countries there are some important exceptions to this trend: on the Indian subcontinent and in other parts of Asia it remains one of the most common forms of cancer. This review article summarises the global incidence of mouth cancer using cancer maps. Data have been compiled from the latest edition of Cancer Incidence in Five Continents and recent studies from various locations around the world. Significant geographic variation is noted in the incidence of mouth cancer, with high rates reported for the Indian subcontinent and parts of Asia (male incidence rates in excess of 10 per 100,000 per annum). It is also noted that as with other forms of oral cancer, the majority of population-based data for mouth cancer comes from the Western world with a paucity of reliable data from the so-called developing countries. Mouth cancer remains a serious health problem in many parts of the world with many regions reporting increasing incidence rates particularly in males. Ongoing research into the aetiologic risk factors associated with this disease must remain a very high priority if the causes of mouth cancer are to be established and disease control protocols introduced widely.
            Bookmark
            • Record: found
            • Abstract: found
            • Article: not found

            Observer variability in the histologic assessment of oral premalignant lesions.

            Histopathologic examination of oral leukoplakias has a major impact on the assessment of prognosis and treatment planning. We investigated the extent of agreement in grading epithelial dysplasia between pathologists with the same or different educational backgrounds. Two general pathologists and two oral pathologists were each given 100 sections of oral leukoplakia to grade from no dysplasia to carcinoma in-situ. The interobserver agreement rates were in the range of 49% to 69%. The calculated kappa values were in the range of 27% to 45%, showing poor to moderate agreement between the pathologists. When comparing the kappa values between the two pairs of pathologists with the same education, these values did not diverge from the general level of kappa values, indicating that the interobserver variability was due to individual differences rather than to educational background.
              Bookmark
              • Record: found
              • Abstract: found
              • Article: not found

              Histological grading of oral epithelial dysplasia: revisited.

              Treatment of oral precancer is largely based on histological grading of epithelial dysplasia, despite the fact that this estimation is subjective and therefore carries a low reproducibility. The grade of epithelial dysplasia may not be proportional to the risk of malignant potential and clinical characteristics may complement therapeutic decisions. Molecular genetic changes found in oral epithelial dysplasia remain unclear and at present lack clinical significance. Genomic and proliferation markers are likely to be associated with histopathological parameters, but their relationship with the grading of dysplasia remains uncertain. They are potential biomarker candidates but their utility in prognosis of oral precancer deserves further study. Copyright 2001 John Wiley & Sons, Ltd.
                Bookmark

                Author and article information

                Journal
                J Oral Maxillofac Pathol
                JOMFP
                Journal of Oral and Maxillofacial Pathology : JOMFP
                Medknow Publications (India )
                0973-029X
                1998-393X
                Jan-Apr 2011
                : 15
                : 1
                : 26-33
                Affiliations
                Department of Oral and Maxillofacial Pathology, V. S. Dental College and Hospital, K. R. Road, VV Puram, Bangalore, India
                [1 ] AECS Maruti College of Dental Sciences and Research Centre, Bannerghatta Road, Bangalore, India
                [2 ] Rajarajeshwari Dental College, Kumbalgodu, Ramohalli Cross, Bangalore, India
                Author notes
                Address for correspondence: Dr. Smitha T, Department of Oral and Maxillofacial Pathology, V. S. Dental College and Hospital, K. R. Road, VV Puram, Bangalore, India. E-mail: dr.smitha.t@ 123456gmail.com
                Article
                JOMFP-15-26
                10.4103/0973-029X.80034
                3125652
                21731274
                282d3b11-92ff-4b99-af08-f1a677bcbf26
                © Journal of Oral and Maxillofacial Pathology

                This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

                History
                Categories
                Original Article

                Pathology
                parameters,leukoplakia,morphometry,image analysis
                Pathology
                parameters, leukoplakia, morphometry, image analysis

                Comments

                Comment on this article