Comparison of Rapid Antigen Tests for COVID-19

There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

Abstract

Reverse transcription-quantitative PCR (RT-qPCR)-based tests are widely used to diagnose coronavirus disease 2019 (COVID-19). As a result that these tests cannot be done in local clinics where RT-qPCR testing capability is lacking, rapid antigen tests (RATs) for COVID-19 based on lateral flow immunoassays are used for rapid diagnosis. However, their sensitivity compared with each other and with RT-qPCR and infectious virus isolation has not been examined. Here, we compared the sensitivity among four RATs by using severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) isolates and several types of COVID-19 patient specimens and compared their sensitivity with that of RT-qPCR and infectious virus isolation. Although the RATs read the samples containing large amounts of virus as positive, even the most sensitive RAT read the samples containing small amounts of virus as negative. Moreover, all RATs tested failed to detect viral antigens in several specimens from which the virus was isolated. The current RATs will likely miss some COVID-19 patients who are shedding infectious SARS-CoV-2.

Related collections

Most cited references 12

Record: found
Abstract: found
Article: not found

A Novel Coronavirus from Patients with Pneumonia in China, 2019

Na Zhu, Dingyu Zhang, Wenling Wang … (2020)

Summary In December 2019, a cluster of patients with pneumonia of unknown cause was linked to a seafood wholesale market in Wuhan, China. A previously unknown betacoronavirus was discovered through the use of unbiased sequencing in samples from patients with pneumonia. Human airway epithelial cells were used to isolate a novel coronavirus, named 2019-nCoV, which formed a clade within the subgenus sarbecovirus, Orthocoronavirinae subfamily. Different from both MERS-CoV and SARS-CoV, 2019-nCoV is the seventh member of the family of coronaviruses that infect humans. Enhanced surveillance and further investigation are ongoing. (Funded by the National Key Research and Development Program of China and the National Major Project for Control and Prevention of Infectious Disease in China.)

0 comments Cited 12704 times – based on 0 reviews      Review now

Bookmark

Record: found
Abstract: found
Article: found

Interpreting Diagnostic Tests for SARS-CoV-2

Nandini Sethuraman, Sundararaj Jeremiah, Akihide Ryo (2020)

0 comments Cited 760 times – based on 0 reviews      Review now

Bookmark

Record: found
Abstract: found
Article: found

Is Open Access

Enhanced isolation of SARS-CoV-2 by TMPRSS2-expressing cells

Shutoku Matsuyama, Naganori Nao, Kazuya Shirato … (2020)

A novel betacoronavirus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which caused a large respiratory outbreak in Wuhan, China in December 2019, is currently spreading across many countries globally. Here, we show that a TMPRSS2-expressing VeroE6 cell line is highly susceptible to SARS-CoV-2 infection, making it useful for isolating and propagating SARS-CoV-2. Our results reveal that, in common with SARS- and Middle East respiratory syndrome-CoV, SARS-CoV-2 infection is enhanced by TMPRSS2.

0 comments Cited 714 times – based on 0 reviews      Review now

Bookmark

All references

Author and article information

Contributors

Giorgio Palù: Role: Academic Editor

Journal

Journal ID (nlm-ta): Viruses

Journal ID (iso-abbrev): Viruses

Journal ID (publisher-id): viruses

Title: Viruses

Publisher: MDPI

ISSN (Electronic): 1999-4915

Publication date (Electronic): 10 December 2020

Publication date Collection: December 2020

Volume: 12

Issue: 12

Electronic Location Identifier: 1420

Affiliations

[1 ]Division of Virology, Department of Microbiology and Immunology, Institute of Medical Science, The University of Tokyo, Tokyo 108-8639, Japan; ytsakai@ 123456g.ecc.u-tokyo.ac.jp (Y.S.-T.); kk187321@ 123456stu-cbms.k.u-tokyo.ac.jp (Y.F.); yasuhara@ 123456ims.u-tokyo.ac.jp (A.Y.); m-ujie@ 123456ims.u-tokyo.ac.jp (M.U.); syamada@ 123456g.ecc.u-tokyo.ac.jp (S.Y.); ito-mu@ 123456ims.u-tokyo.ac.jp (M.I.); kenken@ 123456ims.u-tokyo.ac.jp (K.I.-H.); mimai@ 123456ims.u-tokyo.ac.jp (M.I.)

[2 ]Division of Infectious Diseases, Advanced Clinical Research Center, Institute of Medical Science, The University of Tokyo, Tokyo 108-8639, Japan; michiko@ 123456ims.u-tokyo.ac.jp (M.K.); saito-id@ 123456ims.u-tokyo.ac.jp (M.S.); kikeuchi004@ 123456gmail.com (K.I.); yotsudid@ 123456ims.u-tokyo.ac.jp (H.Y.)

[3 ]Department of Infectious Diseases and Applied Immunology, IMSUT Hospital of Institute of Medical Science, The University of Tokyo, Tokyo 108-8639, Japan; e-adachi@ 123456ims.u-tokyo.ac.jp (E.A.); nagai.hir@ 123456gmail.com (H.N.)

[4 ]Emergency Medical Center, Fujisawa City Hospital, Kanagawa 251-8550, Japan; samukun036@ 123456gmail.com

[5 ]Pulmonary Division, Department of Internal Medicine, Saiseikai Utsunomiya Hospital, Tochigi 321-0974, Japan; nichiro4747@ 123456gmail.com (I.N.); rie.amechan@ 123456gmail.com (R.B.)

[6 ]Division of Pulmonary Medicine, Department of Internal Medicine, Tachikawa Hospital, Tokyo 190-8531, Japan; h.koh@ 123456tachikawa-hosp.gr.jp (H.K.); ftakahiro0529@ 123456yahoo.co.jp (T.F.); fito102351@ 123456gmail.com (F.I.)

[7 ]Department of Refractory Viral Infections, National Center for Global Health and Medicine Research Institute, Tokyo 162-8655, Japan; kmaeda@ 123456ri.ncgm.go.jp (K.M.); shattori@ 123456ri.ncgm.go.jp (S.-i.H.); hmitsuya@ 123456hosp.ncgm.go.jp (H.M.)

[8 ]Department of Emergency Medicine and Critical Care Medicine, Saiseikai Utsunomiya Hospital, Tochigi 321-0974, Japan; alongthelongestway2003@ 123456yahoo.co.jp (T.O.); fujitak.727@ 123456gmail.com (K.F.)

[9 ]Disease Control and Prevention Center, National Center for Global Health and Medicine Hospital, Tokyo 162-8655, Japan; kyamamoto@ 123456hosp.ncgm.go.jp (K.Y.); tnakamoto@ 123456hosp.ncgm.go.jp (T.N.); nohmagari@ 123456hosp.ncgm.go.jp (N.O.)

[10 ]Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, WI 53706, USA

[11 ]International Research Center for Infectious Diseases, Department of Special Pathogens, Institute of Medical Science, The University of Tokyo, Tokyo 108-8639, Japan

Author notes

[* ]Correspondence: yamayo@ 123456ims.u-tokyo.ac.jp (S.Y.); yoshihiro.kawaoka@ 123456wisc.edu (Y.K.)

Author information

Seiya Yamayoshi https://orcid.org/0000-0001-7768-5157

Yuko Sakai-Tagawa https://orcid.org/0000-0002-7261-4648

Michiko Koga https://orcid.org/0000-0002-3122-9735

Eisuke Adachi https://orcid.org/0000-0002-1623-873X

Rie Baba https://orcid.org/0000-0002-8255-1978

Fumimaro Ito https://orcid.org/0000-0002-1868-6405

Article

Publisher ID: viruses-12-01420

DOI: 10.3390/v12121420

PMC ID: 7764512

PubMed ID: 33322035

SO-VID: 28cd0d3a-b99d-4eba-9b45-33157992588b

License:

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license ( http://creativecommons.org/licenses/by/4.0/).

Comparison of Rapid Antigen Tests for COVID-19

Read this article at

Abstract

Related collections

Novel Coronavirus Disease COVID-19

Most cited references 12

A Novel Coronavirus from Patients with Pneumonia in China, 2019

Interpreting Diagnostic Tests for SARS-CoV-2

Enhanced isolation of SARS-CoV-2 by TMPRSS2-expressing cells

Author and article information

Contributors

Journal

Affiliations

Author notes

Author information

Article

History

Categories

Comments

Comment on this article

Similar content 170

Cited by 91

Most referenced authors 481