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      Radioimmunoassay for Human Pituitary Prolactin, Using Antiserum against an Extract of Human Amniotic Fluid


      Hormone Research in Paediatrics

      S. Karger AG

      Amniotic fluid, Prolactin, Radioimmunoassay, Human

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          Rabbit antiserum raised against a prolactin-rich extract of human amniotic fluid was used to establish a double-antibody radioimmunoassay for human pituitary prolactin inplasma. The binding of <sup>131</sup>I-labelIed human pituitary prolactin(Lewis) to the antiserum was inhibited by unlabelled prolactin, human chorionic gonadotrophin (HCG), human luteinizing hormone (HLH) and human follicle stimulating hormone (HFSH). Absorption of the antiserum with HCG rendered the assay specific for prolactin no cross-reaction being then observed with HLH, HFSH, human thyroid stimulating hormone, human growth hormone or human chorionic somatomammotrophin. The binding of <sup>131</sup>I-labelled human pituitary prolactin (Friesen) to antibody was not affected by HCG. The concentration of prolactin in plasmas from normal males, pregnant females, non-pregnant females and in patients treated with phenothiazine was measured.

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          Author and article information

          Horm Res Paediatr
          Hormone Research in Paediatrics
          S. Karger AG
          21 November 2008
          : 4
          : 5
          : 261-273
          Tenovus Institute for Cancer Research and Department of Chemical Pathology, Welsh National School of Medicine, Cardiff
          178313 Horm Res 1973;4:261–273
          © 1973 S. Karger AG, Basel

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          Page count
          Pages: 13


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