Generation of Definitive Neural Progenitor Cells from Human Pluripotent Stem Cells for Transplantation into Spinal Cord Injury.

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Abstract

In this chapter, we first describe two interchangeable protocols optimized in our lab for deriving definitive neuronal progenitor cells from human pluripotent stem cells (hPSCs). The resultant NPCs can then be propagated and differentiated to produce differing proportions of neurons, oligodendrocytes, and astrocytes as required for in vitro cell culture studies or in vivo transplantation. Following these protocols, we explain the method for transplanting these cells into the rat model of spinal cord injury (SCI).

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Journal

Journal ID (iso-abbrev): Methods Mol Biol

Title: Methods in molecular biology (Clifton, N.J.)

Publisher: Springer Science and Business Media LLC

ISSN (Electronic): 1940-6029

ISSN (Print): 1064-3745

Publication date (Electronic): 2019

Volume: 1919

Affiliations

[1 ] Division of Genetics and Development, Krembil Research Institute, Toronto, ON, Canada.

[2 ] Institute of Medical Science, University of Toronto, Toronto, ON, Canada.

[3 ] Division of Neurosurgery, University of Toronto, Toronto, ON, Canada.

[4 ] Division of Genetics and Development, Krembil Research Institute, Toronto, ON, Canada. Michael.Fehlings@uhn.ca.

[5 ] Institute of Medical Science, University of Toronto, Toronto, ON, Canada. Michael.Fehlings@uhn.ca.

[6 ] Division of Neurosurgery, University of Toronto, Toronto, ON, Canada. Michael.Fehlings@uhn.ca.

[7 ] Spinal Program, Toronto Western Hospital, University Health Network, Toronto, ON, Canada. Michael.Fehlings@uhn.ca.

[8 ] Faculty of Medicine, University of Toronto, Toronto, ON, Canada. Michael.Fehlings@uhn.ca.

Article

DOI: 10.1007/978-1-4939-9007-8_3

PubMed ID: 30656619

SO-VID: 28f81b5f-e688-43d9-90e8-972d872a89b6

History

Keywords: Pluripotent stem cells,Spinal cord injury,Neural progenitor cells,Cell transplantation

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Keywords: Pluripotent stem cells, Spinal cord injury, Neural progenitor cells, Cell transplantation

Generation of Definitive Neural Progenitor Cells from Human Pluripotent Stem Cells for Transplantation into Spinal Cord Injury.

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