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      Silver, gold and bimetallic nanoparticles production using single-cell protein (Spirulina platensis) Geitler

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          Nanoparticles with Raman spectroscopic fingerprints for DNA and RNA detection.

          Multiplexed detection of oligonucleotide targets has been performed with gold nanoparticle probes labeled with oligonucleotides and Raman-active dyes. The gold nanoparticles facilitate the formation of a silver coating that acts as a surface-enhanced Raman scattering promoter for the dye-labeled particles that have been captured by target molecules and an underlying chip in microarray format. The strategy provides the high-sensitivity and high-selectivity attributes of gray-scale scanometric detection but adds multiplexing and ratioing capabilities because a very large number of probes can be designed based on the concept of using a Raman tag as a narrow-band spectroscopic fingerprint. Six dissimilar DNA targets with six Raman-labeled nanoparticle probes were distinguished, as well as two RNA targets with single nucleotide polymorphisms. The current unoptimized detection limit of this method is 20 femtomolar.
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            Biosynthesis of silver and gold nanoparticles by novel sundried Cinnamomum camphora leaf

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              Quantum-dot-tagged microbeads for multiplexed optical coding of biomolecules.

              Multicolor optical coding for biological assays has been achieved by embedding different-sized quantum dots (zinc sulfide-capped cadmium selenide nanocrystals) into polymeric microbeads at precisely controlled ratios. Their novel optical properties (e.g., size-tunable emission and simultaneous excitation) render these highly luminescent quantum dots (QDs) ideal fluorophores for wavelength-and-intensity multiplexing. The use of 10 intensity levels and 6 colors could theoretically code one million nucleic acid or protein sequences. Imaging and spectroscopic measurements indicate that the QD-tagged beads are highly uniform and reproducible, yielding bead identification accuracies as high as 99.99% under favorable conditions. DNA hybridization studies demonstrate that the coding and target signals can be simultaneously read at the single-bead level. This spectral coding technology is expected to open new opportunities in gene expression studies, high-throughput screening, and medical diagnostics.
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                Author and article information

                Journal
                Journal of Materials Science
                J Mater Sci
                Springer Nature
                0022-2461
                1573-4803
                August 2008
                June 7 2008
                August 2008
                : 43
                : 15
                : 5115-5122
                Article
                10.1007/s10853-008-2745-4
                2932b1c8-73a8-485e-88c3-e386d0b2dd1e
                © 2008
                History

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