The minimal cadherin-catenin complex binds to actin filaments under force

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Abstract

Linkage between the adherens junction (AJ) and the actin cytoskeleton is required for tissue development and homeostasis. In vivo findings indicated that the AJ proteins E-cadherin, β-catenin, and the filamentous (F)-actin binding protein αE-catenin form a minimal cadherin-catenin complex that binds directly to F-actin. Biochemical studies challenged this model because the purified cadherin-catenin complex does not bind F-actin in solution. Here, we reconciled this difference. Using an optical trap-based assay, we showed that the minimal cadherin-catenin complex formed stable bonds with an actin filament under force. Bond dissociation kinetics can be explained by a catch-bond model in which force shifts the bond from a weakly to a strongly bound state. These results may explain how the cadherin-catenin complex transduces mechanical forces at cell-cell junctions. Copyright © 2014, American Association for the Advancement of Science.

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Author and article information

Journal

Title: Science

Abbreviated Title: Science

Publisher: American Association for the Advancement of Science (AAAS)

ISSN (Print): 0036-8075

ISSN (Electronic): 1095-9203

Publication date Created: October 30 2014

Publication date Created: October 31 2014

Publication date (Electronic): October 30 2014

Publication date (Print): October 31 2014

Volume: 346

Issue: 6209

Page: 1254211

Article

DOI: 10.1126/science.1254211

PMC ID: 4364042

PubMed ID: 25359979

SO-VID: 29bdb55d-7f84-4b90-bb24-98def8622c8a

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