99
views
0
recommends
+1 Recommend
1 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: found

      Genetic variation among species, races, forms and inbred lines of lac insects belonging to the genus Kerria (Homoptera, Tachardiidae)

      research-article

      Read this article at

      ScienceOpenPublisherPMC
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          The lac insects (Homoptera: Tachardiidae), belonging to the genus Kerria, are commercially exploited for the production of lac. Kerria lacca is the most commonly used species in India. RAPD markers were used for assessing genetic variation in forty-eight lines of Kerria, especially among geographic races, infrasubspecific forms, cultivated lines, inbred lines, etc., of K. lacca. In the 48 lines studied, the 26 RAPD primers generated 173 loci, showing 97.7% polymorphism. By using neighbor-joining, the dendrogram generated from the similarity matrix resolved the lines into basically two clusters and outgroups. The major cluster, comprising 32 lines, included mainly cultivated lines of the rangeeni form, geographic races and inbred lines of K. lacca. The second cluster consisted of eight lines of K. lacca, seven of the kusmi form and one of the rangeeni from the southern state of Karnataka. The remaining eight lines formed a series of outgroups, this including a group of three yellow mutant lines of K. lacca and other species of the Kerria studied, among others. Color mutants always showed distinctive banding patterns compared to their wild-type counterparts from the same population. This study also adds support to the current status of kusmi and rangeeni, as infraspecific forms of K. lacca.

          Related collections

          Most cited references107

          • Record: found
          • Abstract: found
          • Article: not found

          Estimation of average heterozygosity and genetic distance from a small number of individuals.

          M Nei (1978)
          The magnitudes of the systematic biases involved in sample heterozygosity and sample genetic distances are evaluated, and formulae for obtaining unbiased estimates of average heterozygosity and genetic distance are developed. It is also shown that the number of individuals to be used for estimating average heterozygosity can be very small if a large number of loci are studied and the average heterozygosity is low. The number of individuals to be used for estimating genetic distance can also be very small if the genetic distance is large and the average heterozygosity of the two species compared is low.
            Bookmark
            • Record: found
            • Abstract: found
            • Article: not found

            DNA polymorphisms amplified by arbitrary primers are useful as genetic markers.

            Molecular genetic maps are commonly constructed by analyzing the segregation of restriction fragment length polymorphisms (RFLPs) among the progeny of a sexual cross. Here we describe a new DNA polymorphism assay based on the amplification of random DNA segments with single primers of arbitrary nucleotide sequence. These polymorphisms, simply detected as DNA segments which amplify from one parent but not the other, are inherited in a Mendelian fashion and can be used to construct genetic maps in a variety of species. We suggest that these polymorphisms be called RAPD markers, after Random Amplified Polymorphic DNA.
              Bookmark
              • Record: found
              • Abstract: found
              • Article: not found

              Fingerprinting genomes using PCR with arbitrary primers.

              Simple and reproducible fingerprints of complex genomes can be generated using single arbitrarily chosen primers and the polymerase chain reaction (PCR). No prior sequence information is required. The method, arbitrarily primed PCR (AP-PCR), involves two cycles of low stringency amplification followed by PCR at higher stringency. We show that strains can be distinguished by comparing polymorphisms in genomic fingerprints. The generality of the method is demonstrated by application to twenty four strains from five species of Staphylococcus, eleven strains of Streptococcus pyogenes and three varieties of Oryza sativa (rice).
                Bookmark

                Author and article information

                Journal
                Genet Mol Biol
                GMB
                Genetics and Molecular Biology
                Sociedade Brasileira de Genética (Ribeirão Preto, SP, Brazil )
                1415-4757
                1678-4685
                1 July 2011
                Jul-Sep 2011
                : 34
                : 3
                : 511-519
                Affiliations
                [1 ]Lac Production Division, Indian Institute of Natural Resins and Gums, Indian Council of Agricultural Research, Ranchi, India.
                [2 ]National Bureau of Plant Genetic Resources, New Delhi, India.
                [3 ]Department of Biotechnology, Birla Institute of Technology, Mesra, India.
                Author notes
                Send correspondence to Ranganathan Ramani. Indian Institute of Natural Resins and Gums, Ranchi 834010 Jharkhand, India. E-mail: ramani_9@ 123456scientist.com .
                Article
                gmb-34-3-511
                10.1590/S1415-47572011000300023
                3168195
                21931527
                2a0b79cb-648b-4c9f-b546-fdc92932c7f9
                Copyright © 2011, Sociedade Brasileira de Genética. Printed in Brazil

                License information: This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

                History
                : 7 February 2011
                : 4 May 2011
                Categories
                Evolutionary Genetics
                Research Article

                Molecular biology
                genetic variation,lac insects,rapd,tachardiidae,kerria,dna fingerprinting
                Molecular biology
                genetic variation, lac insects, rapd, tachardiidae, kerria, dna fingerprinting

                Comments

                Comment on this article

                scite_

                Similar content56

                Cited by5

                Most referenced authors574