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      Advanced oxidation protein product levels as a marker of oxidative stress in paediatric patients with chronic tonsillitis Translated title: Ruolo dei prodotti avanzati di ossidazione proteica come marker di stress ossidativo nei pazienti pediatrici affetti da tonsillite cronica

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          SUMMARY

          We aimed to determine whether advanced oxidation protein product (AOPP) levels can serve as a marker of oxidative stress in paediatric patients with chronic tonsillitis. Thirty children with chronic tonsillitis and 30 healthy children (control group) were recruited from the Otorhinolaryngology (ORL) and Paediatric Surgery departments, respectively, of Dumlupinar University Hospital. In the patient group, blood samples were collected before tonsillectomy, and tonsil tissue was sampled during the operation. Blood samples were also obtained from the control subjects. AOPP levels in the serum and tonsil tissue were measured by the spectrophotometric method. Serum AOPP levels were significantly higher in the patient group (13.1 ± 3.3 ng/ml) than in the control group (11.6 ± 2.3 ng/ml; P < 0.05). In addition, the mean AOPP level (41.9 ± 13.5 ng/mg protein) in the tonsil tissue in the patient group was significantly higher than the mean serum AOPP levels in the control and patient groups (P < 0.05). AOPP levels are elevated in the tonsil tissue and serum of patients with chronic tonsillitis compared to the serum AOPP levels in healthy controls. AOPPs may represent a novel class of pro-inflammatory molecules that are involved in oxidative stress in chronic tonsillitis. AOPPs may be used as a marker of oxidative stress in paediatric patients with chronic tonsillitis.

          RIASSUNTO

          L'obiettivo del presente studio è stato determinare se i livelli plasmatici dei prodotti avanzati di ossidazione proteica (AOPP) rappresentino dei marker di stress ossidativo nei pazienti pediatrici affetti da tonsillite cronica. Per lo studio sono stati arruolati, presso i Dipartimenti di Otorinolaringoiatria e Chirurgia pediatrica dell'Ospedale Universitario di Dumlupinar, trenta bambini sani e trenta affetti da tonsillite cronica. Il gruppo dei pazienti affetti da malattia è stato sottoposto a un prelievo ematico preoperatorio e ad una biopsia intraoperatoria del tessuto tonsillare. Il gruppo dei pazienti sani è stato sottoposto unicamente al prelievo ematico. I livelli plasmatici e tissutali degli AOPP sono quindi stati misurati mediante spettrofotometria. I livelli sierici degli AOPP sono risultati essere più elevati nel gruppo dei pazienti affetti da tonsillite cronica (13,1 ± 3,3 ng/ml) rispetto al gruppo di controllo (11,6 ± 2,3 ng/ml; P < 0,05). Il livello tissutale medio degli AOPP nei pazienti malati è risultato essere superiore a quello plasmatico medio sia nel gruppo dei pazienti sani che in quello dei pazienti malati (41,9 ± 13,5 ng/mg; P < 0,05). I livelli plasmatici e tissutali degli AOPP sono risultati quindi essere più elevati nei pazienti malati rispetto al gruppo di controllo. Gli AOPP potrebbero quindi rappresentare una nuova classe di molecole pro-infiammatorie coinvolte nello stress ossidativo nella tonsillite cronica e potrebbero avere un ruolo come marker di stress ossidativo nei pazienti pediatrici affetti da tale patologia.

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            Oxidant species such as superoxide radical (O.2-), hydrogen peroxide (H2O2), hydroxyl radical (HO.), and lipid peroxides (LOOH) are becoming increasingly implicated in human disease. However, the question of whether such oxidants are a major cause of tissue injury in human disease or are merely produced during such injury has been difficult to answer because of inadequate experimental techniques, and possibly because of an overemphasis on lipid peroxidation as a mechanism of oxidant injury. Recent developments in methodology, in our understanding of the primary mechanism of oxidant toxicity to cells, and in concepts of antioxidant protection are reviewed. Good evidence now exists for some role of oxidant damage to tissues in the pathology of several human diseases, including rheumatoid arthritis, reperfusion injury, immune injury to lung and kidney, and cerebral trauma or ischemia. These have led to promising suggestions for new therapeutic approaches.
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              Oxidative stress in critically ill patients.

              Oxygen-derived free radicals play an important role in the development of disease in critically ill patients. Normally, oxygen free radicals are neutralized by antioxidants such as vitamin E or enzymes such as superoxide dismutase. However, in patients who require intensive care, oxygen free radicals become a problem when either a decrease in the removal or an overproduction of the radicals occurs. This oxidative stress and the damage due to it have been implicated in many diseases in critically ill patients. Many drugs and treatments now being investigated are directed toward preventing the damage from oxidative stress. The formation of reactive oxygen species, the damage caused by them, and the body's defense system against them are reviewed. New interventions are described that may be used in critically ill patients to prevent or treat oxidative damage.
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                Author and article information

                Journal
                Acta Otorhinolaryngol Ital
                Acta Otorhinolaryngol Ital
                Pacini
                Acta Otorhinolaryngologica Italica
                Pacini Editore SRL
                0392-100X
                1827-675X
                October 2016
                : 36
                : 5
                : 381-385
                Affiliations
                [1 ] Department of ORL, Dumlupınar University, Kutahya, Turkey;
                [2 ] Department of Otolaryngology-Head and Neck Surgery, "Arthur G. James" Cancer Hospital and "Richard J. Solove" Research Institute, Comprehensive Cancer Center, the Ohio State University Wexner Medical Center, Columbus, Ohio, USA;
                [3 ] Department of Biochemistry, Dumlupınar University, Kutahya, Turkey;
                [4 ] Department of Pediatric Surgery, Dumlupınar University, Kutahya, Turkey
                Author notes
                Address for correspondence: Isa Ozbay, Department of Otorhinolaryngology, Faculty of Medicine, Dumlupinar University, 43000 Kutahya, Turkey. Tel. +9005059311869. Fax +9002742316673. Email: isaozbay@ 123456yahoo.com
                Article
                Pacini
                10.14639/0392-100X-897
                5225793
                27958598
                2a248b58-6d3e-4b65-bb79-5a3ae0ad8256
                © Copyright by Società Italiana di Otorinolaringologia e Chirurgia Cervico-Facciale, Rome, Italy

                This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives License, which permits for noncommercial use, distribution, and reproduction in any digital medium, provided the original work is properly cited and is not altered in any way. For details, please refer to http://creativecommons.org/licenses/by-nc-nd/3.0/

                History
                : 26 October 2015
                : 26 March 2016
                Categories
                Paediatric Otorhinolaryngology

                Otolaryngology
                chronic tonsillitis,advanced oxidation protein products (aopps),oxidative stress

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