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      Mechanisms of cell death in canine parvovirus-infected cells provide intuitive insights to developing nanotools for medicine

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          Abstract

          Viruses have great potential as nanotools in medicine for gene transfer, targeted gene delivery, and oncolytic cancer virotherapy. Here we have studied cell death mechanisms of canine parvovirus (CPV) to increase the knowledge on the CPV life cycle in order to facilitate the development of better parvovirus vectors. Morphological studies of CPV-infected Norden laboratory feline kidney (NLFK) cells and canine fibroma cells (A72) displayed characteristic apoptotic events. Apoptosis was further confirmed by activation of caspases and cellular DNA damage. However, results from annexin V-propidium iodide (PI) labeling and membrane polarization assays indicated disruption of the plasma membrane uncommon to apoptosis. These results provide evidence that secondary necrosis followed apoptosis. In addition, two human cancer cell lines were found to be infected by CPV. This necrotic event over apoptotic cell death and infection in human cells provide insightful information when developing CPV as a nanotool for cancer treatments.

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          Author and article information

          Journal
          Int J Nanomedicine
          International Journal of Nanomedicine
          Dove Medical Press
          1176-9114
          1178-2013
          2010
          2010
          9 August 2010
          : 5
          : 417-428
          Affiliations
          Nanoscience Center and Department of Biological and Environmental Science, University of Jyväskylä, Finland
          Author notes
          Correspondence: Leona Gilbert, PO Box 35, FI-40014 University of Jyväskylä, Finland, Tel +358 14 260 4208, Fax +358 14 260 2321, Email leona.k.gilbert@ 123456jyu.fi
          Article
          ijn-5-417
          2950399
          20957163
          © 2010 Nykky et al, publisher and licensee Dove Medical Press Ltd.

          This is an Open Access article which permits unrestricted noncommercial use, provided the original work is properly cited.

          Categories
          Original Research

          Molecular medicine

          apoptosis, necrosis, nanoparticle, canine parvovirus, virotherapy

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