14
views
0
recommends
+1 Recommend
0 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: not found

      miR-130a regulates differential lipid accumulation between intramuscular and subcutaneous adipose tissues of pigs via suppressing PPARG expression.

      Read this article at

      ScienceOpenPublisherPubMed
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          To investigate the molecular mechanism underlying differential lipid deposition between intramuscular (IM) and subcutaneous (SC) fat tissues of pigs, peroxisome proliferator-activated receptor gamma (PPARG) expression levels were compared in these two types of adipose tissues. The results showed that both mRNA and protein levels of PPARG were significantly higher in SC fat than in IM fat. Correspondingly, the expression levels of miR-128 and miR-130a, which potentially targeting PPARG 3'-untranslated region (3'-UTR), in IM fat were 8.37 and 2.30-fold of those in SC fat respectively. Further dual luciferase activity assay showed that only miR-130a directly targeting PPARG. Moreover, over-expression of miR-130a in preadipocytes significantly inhibited its differentiation by suppressing PPARG expression. In conclusion, tissue variance of miR-130a levels results in the diverse of PPARG, and might be the reason for differential fat deposition between IM and SC fat tissues in pigs. Our study would provide the molecular foundation for IM fat deposition increase and therefore contribute to pork quality improvement.

          Related collections

          Author and article information

          Journal
          Gene
          Gene
          Elsevier BV
          1879-0038
          0378-1119
          Dec 15 2017
          : 636
          Affiliations
          [1 ] College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China.
          [2 ] College of Public Health, Nantong University, Nantong, Jiangsu 226019, China.
          [3 ] College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China. Electronic address: jiechen@njau.edu.cn.
          Article
          S0378-1119(17)30674-1
          10.1016/j.gene.2017.08.036
          28882569
          2ac39560-19e8-405e-870b-005325e72ea3
          History

          miR-130a,PPARG,Fat deposition,Pork quality
          miR-130a, PPARG, Fat deposition, Pork quality

          Comments

          Comment on this article