47
views
0
recommends
+1 Recommend
0 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: found
      Is Open Access

      Highly Sensitive Immunochromatographic Identification of Tetracycline Antibiotics in Milk

      research-article

      Read this article at

      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          A rapid immunochromatographic assay was developed for the control of tetracycline (TC). The assay is based on the competition between immobilized TC-protein conjugate and TC in a tested sample for binding with polyclonal anti-TC antibodies conjugated to colloidal gold during the flow of the sample along a membrane strip with immobilized reactants. Conjugation of colloidal gold and the total immunoglobulin (IgG) fraction of polyclonal antibodies was used to increase the assay sensitivity to ensure low content of specific antibodies in the conjugate. This allowed effective inhibition of free TC and conjugate binding in the strip test zone. Photometric marker registration allows control of the reduction of binding, thereby enhancing detection sensitivity. The proposed assay allows TC to be detected at concentrations up to 20 ng/mL, exceeding the limit of detection of the known analogues, in a wide working range (more than two orders) of 60 pg/mL to 10 ng/mL, ensured through the use of polyclonal antibodies. The assay time is 10 min. The efficiency of the designed assay is shown to identify TC in milk; the degree of recovery of TC ranges from 90 to 112%. The precision of the concentrations measurements was no more than 10%.

          Related collections

          Most cited references42

          • Record: found
          • Abstract: not found
          • Article: not found

          Physicochemical Properties and Cellular Toxicity of Nanocrystal Quantum Dots Depend on Their Surface Modification

            Bookmark
            • Record: found
            • Abstract: found
            • Article: not found

            Enhanced lateral flow immunoassay using gold nanoparticles loaded with enzymes.

            The use of gold nanoparticles (AuNPs) as labeling carriers in combination with the enzymatic activity of the horseradish peroxidase (HRP) in order to achieve an improved optical lateral flow immunoassay (LFIA) performance is presented here. Briefly in a LFIA with an immune-sandwich format AuNPs are functionalized with a detection antibody already modified with HRP, obtaining an 'enhanced' label. Two different detection strategies have been tested: the first one following just the red color of the AuNPs and the second one using a substrate for the HRP (3 different substrates are evaluated), which produces a darker color that enhances the intensity of the previous red color of the unmodified AuNPs. In such very simple way it is gaining sensitivity (up to 1 order of magnitude) without losing the simplicity of the LFIA format, opening the way to other LFIA applications including their on-demand performance tuning according to the analytical scenario.
              Bookmark
              • Record: found
              • Abstract: not found
              • Book Chapter: not found

              Front-matter

              (2013)
                Bookmark

                Author and article information

                Journal
                Int J Anal Chem
                Int J Anal Chem
                IJAC
                International Journal of Analytical Chemistry
                Hindawi Publishing Corporation
                1687-8760
                1687-8779
                2015
                25 November 2015
                : 2015
                : 347621
                Affiliations
                1A.N. Bach Institute of Biochemistry, Research Centre of Biotechnology of the Russian Academy of Sciences, Leninsky Prospect 33, Moscow 119071, Russia
                2Institute of Physical Organic Chemistry, Surganov Street 13, 220072 Minsk, Belarus
                3Institute of Bioorganic Chemistry, Acad. Kuprevich Street 5/2, 220141 Minsk, Belarus
                4Chemical Department, M.V. Lomonosov Moscow State University, Leninskie Gory, Moscow 119991, Russia
                Author notes

                Academic Editor: Josep Esteve-Romero

                Article
                10.1155/2015/347621
                4673384
                26689537
                2ac9209d-7a06-4c97-8f34-b03b1a925a17
                Copyright © 2015 N. A. Taranova et al.

                This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

                History
                : 17 September 2015
                : 4 November 2015
                Categories
                Research Article

                Analytical chemistry
                Analytical chemistry

                Comments

                Comment on this article