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      Highly Sensitive Immunochromatographic Identification of Tetracycline Antibiotics in Milk

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          Abstract

          A rapid immunochromatographic assay was developed for the control of tetracycline (TC). The assay is based on the competition between immobilized TC-protein conjugate and TC in a tested sample for binding with polyclonal anti-TC antibodies conjugated to colloidal gold during the flow of the sample along a membrane strip with immobilized reactants. Conjugation of colloidal gold and the total immunoglobulin (IgG) fraction of polyclonal antibodies was used to increase the assay sensitivity to ensure low content of specific antibodies in the conjugate. This allowed effective inhibition of free TC and conjugate binding in the strip test zone. Photometric marker registration allows control of the reduction of binding, thereby enhancing detection sensitivity. The proposed assay allows TC to be detected at concentrations up to 20 ng/mL, exceeding the limit of detection of the known analogues, in a wide working range (more than two orders) of 60 pg/mL to 10 ng/mL, ensured through the use of polyclonal antibodies. The assay time is 10 min. The efficiency of the designed assay is shown to identify TC in milk; the degree of recovery of TC ranges from 90 to 112%. The precision of the concentrations measurements was no more than 10%.

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          Physicochemical Properties and Cellular Toxicity of Nanocrystal Quantum Dots Depend on Their Surface Modification

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              Compact cysteine-coated CdSe(ZnCdS) quantum dots for in vivo applications.

              We have developed a versatile nanoparticle construct using a compact cysteine coating on a CdSe(ZnCdS) core(shell) nanocrystal (QD-Cys) that is biologically compatible, exceptionally compact, highly fluorescent, and easily functionalized. The small hydrodynamic diameter of QD-Cys ( approximately 6 nm) allows for renal clearance of these nanoparticles in rat models. Moreover, the ability to directly conjugate to QD-Cys opens up the possibility of functionalized nanocrystals for in vivo targeted imaging, in which small targeting molecules can be appended to QD-Cys, and unbound QDs can be rapidly cleared to achieve high signal/noise ratios and to reduce background toxicity.
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                Author and article information

                Journal
                Int J Anal Chem
                Int J Anal Chem
                IJAC
                International Journal of Analytical Chemistry
                Hindawi Publishing Corporation
                1687-8760
                1687-8779
                2015
                25 November 2015
                : 2015
                Affiliations
                1A.N. Bach Institute of Biochemistry, Research Centre of Biotechnology of the Russian Academy of Sciences, Leninsky Prospect 33, Moscow 119071, Russia
                2Institute of Physical Organic Chemistry, Surganov Street 13, 220072 Minsk, Belarus
                3Institute of Bioorganic Chemistry, Acad. Kuprevich Street 5/2, 220141 Minsk, Belarus
                4Chemical Department, M.V. Lomonosov Moscow State University, Leninskie Gory, Moscow 119991, Russia
                Author notes

                Academic Editor: Josep Esteve-Romero

                Article
                10.1155/2015/347621
                4673384
                Copyright © 2015 N. A. Taranova et al.

                This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

                Categories
                Research Article

                Analytical chemistry

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