Blog
About

5
views
0
recommends
+1 Recommend
1 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: found
      Is Open Access

      Initiation of primary cell cultures from embryos of the mosquitoes Anopheles albimanus and Aedes taeniorhynchus (Diptera: Culicidae)

      Read this article at

      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          Primary cell cultures were obtained from eggs of Anopheles albimanus and Aedes taeniorhynchus mosquitoes, vectors of human malaria and of Venezuelan equine encephalitis virus, respectively. The cellular growth of the An. albimanus cells began four weeks after explanting the embryonic tissues in MK/VP12 medium, supplemented with 15% fetal bovine serum. The culture showed heterogeneous cellular morphology. With regard to the Ae. taeniorhynchus culture, growth occurred three weeks after initiating the culture in MM/VP12 medium. The majority of cells were small and round. Karyotypes were examined in the latter species.

          Related collections

          Author and article information

          Contributors
          Role: ND
          Role: ND
          Role: ND
          Role: ND
          Role: ND
          Journal
          mioc
          Memórias do Instituto Oswaldo Cruz
          Mem. Inst. Oswaldo Cruz
          Instituto Oswaldo Cruz, Ministério da Saúde (Rio de Janeiro )
          1678-8060
          August 1995
          : 90
          : 4
          : 547-551
          Affiliations
          [1 ] Universidad de La Salle Colombia
          [2 ] Instituto Nacional de Salud Colombia
          S0074-02761995000400024
          10.1590/S0074-02761995000400024

          http://creativecommons.org/licenses/by/4.0/

          Product
          Product Information: SciELO Brazil
          Categories
          PARASITOLOGY
          TROPICAL MEDICINE

          Parasitology, Infectious disease & Microbiology

          karyotype, culture media, cell cultures, mosquitoes

          Comments

          Comment on this article