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      Polymerase chain reaction for the evaluation of Schistosoma mansoni infection in two low endemicity areas of Minas Gerais, Brazil.

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          Abstract

          This study aimed to evaluate the occurrence of schistosomiasis in areas with low endemicity using polymerase chain reaction (PCR) as a diagnostic method. We analysed faecal samples from 219 individuals residing in Piau and Coronel Pacheco, state of Minas Gerais, Brazil, using a single faecal sample from each individual and two slides of the Kato-Katz technique as a gold standard. Fifteen out of the 219 samples were positive with both methods of diagnosis. One sample was diagnosed as positive by the Kato-Katz technique only and 61 were diagnosed only by PCR. The positivity rates were 7.3% with the Kato-Katz method and 34.7% with PCR. When both techniques were assumed to have 100% specificity and positive individuals were identified by both methods, the sensitivity of the Kato-Katz method was 20.8% and the PCR sensitivity was 98.7%. The Kappa index between the two techniques was 0.234, suggesting weak agreement. The assessment of a single faecal sample by PCR detected more cases of infection than the analysis of one sample with two slides using the Kato-Katz technique, suggesting that PCR can be a useful diagnostic tool, particularly in areas with low endemicity.

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          Author and article information

          Journal
          Mem. Inst. Oswaldo Cruz
          Memorias do Instituto Oswaldo Cruz
          1678-8060
          0074-0276
          Nov 2012
          : 107
          : 7
          Affiliations
          [1 ] Departamento de Parasitologia, Microbiologia e Imunologia, Universidade Federal de Juiz de Fora, MG, Brasil. gabrielbiomed@hotmail.com
          Article
          S0074-02762012000700010
          23147146
          2b42d180-5fec-49bb-baa3-54943e6c9457

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