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      Fabrication of sharp silicon arrays to wound Caenorhabditis elegans

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          Abstract

          Understanding how animals respond to injury and how wounds heal remains a challenge. These questions can be addressed using genetically tractable animals, including the nematode Caenorhabditis elegans. Given its small size, the current methods for inflicting wounds in a controlled manner are demanding. To facilitate and accelerate the procedure, we fabricated regular arrays of pyramidal features ("pins”) sharp enough to pierce the tough nematode cuticle. The pyramids were made from monocrystalline silicon wafers that were micro-structured using optical lithography and alkaline wet etching. The fabrication protocol and the geometry of the pins, determined by electron microscopy, are described in detail. We also used electron microscopy to characterize the different types of injury caused by these pins. Upon wounding, C. elegans expresses genes encoding antimicrobial peptides. A comparison of the induction of antimicrobial peptide gene expression using traditional needles and the pin arrays demonstrates the utility of this new method.

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          Most cited references24

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          What is the Young's Modulus of Silicon?

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            Microneedle arrays as transdermal and intradermal drug delivery systems: Materials science, manufacture and commercial development

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              Anisotropic Etching of Crystalline Silicon in Alkaline Solutions

              R H Seidel (1990)
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                Author and article information

                Contributors
                ozerov@cinam.univ-mrs.fr
                ewbank@ciml.univ-mrs.fr
                Journal
                Sci Rep
                Sci Rep
                Scientific Reports
                Nature Publishing Group UK (London )
                2045-2322
                27 February 2020
                27 February 2020
                2020
                : 10
                : 3581
                Affiliations
                [1 ]ISNI 0000 0004 0639 5277, GRID grid.417850.f, Aix Marseille Univ, CNRS, INSERM, CIML, Turing Centre for Living Systems, ; Marseille, France
                [2 ]ISNI 0000 0001 2176 4817, GRID grid.5399.6, Aix Marseille Univ, CNRS, CINaM, ; Marseille, France
                Article
                60333
                10.1038/s41598-020-60333-7
                7046703
                32108170
                2b439147-2dd7-4b39-a791-95461af8bb19
                © The Author(s) 2020

                Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

                History
                : 19 September 2019
                : 2 January 2020
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                © The Author(s) 2020

                Uncategorized
                innate immunity,skin models,genetic models,surface patterning,biomedical engineering
                Uncategorized
                innate immunity, skin models, genetic models, surface patterning, biomedical engineering

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