The acute effect of stress on the plasma and cerebrospinal fluid (CSF) immuno-reactive parathyroid hormone (PTH) response was studied in 6 merino sheep. Stress was exerted by weak periodical electric square waves (PESW). In addition, the effect of intravenous injection of adrenaline was studied. Under stress conditions (PESW or adrenaline injection), plasma PTH increased up to 30% and up to 50%, respectively. Weak periodical electric square waves of 3–4 mA decreased CSF PTH concentrations by up to 50%. The effect of adrenaline injection on the CSF PTH was not significant. Total calcium and magnesium in plasma and CSF did not change. Our results showed that the effect of stress on the CSF PTH is opposite to the effect on plasma PTH, and suggest that both, PESW and adrenaline, affected PTH in plasma and CSF by a Ca-independent mechanism.